A doublet-separated sensitivity-enhanced HSQC for the determination of scalar and dipolar one-bond J-couplings

被引：100
作者：
Cordier, F
Dingley, AJ
Grzesiek, S
机构：
[1] Forschungszentrum Julich, Inst Biol Struct, IBI-2, D-52425 Julich, Germany
[2] Univ Dusseldorf, Inst Phys Biol, D-40225 Dusseldorf, Germany
来源：
JOURNAL OF BIOMOLECULAR NMR | 1999年 / 13卷 / 02期
关键词：
dipolar coupling; hepatitis C protease; one-bond J coupling; sensitivity enhancement;
D O I：
10.1023/A:1008301415843
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple, sensitivity-enhanced HSQC experiment is described which separates the upheld and downfield components in the indirect dimension into different subspectra. The sequence is similar to the generalized TROSY scheme; however, decoupling of the X-nucleus is used during detection. A detailed analysis of relaxation effects, precision and sensitivity of the method is presented. The approach is demonstrated in a two-dimensional water flipback (1)H-(15)N HSQC which measures (1)J(HN) splittings in isotropic and oriented samples of ubiquitin and the hepatitis C protease. The results are in excellent agreement with splittings obtained from a conventional (1)H-coupled HSQC.
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收藏
页码：175 / 180
页数：6
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