Be more specific! Laser-assisted microdissection of plant cells

被引:97
作者
Day, RC
Grossniklaus, U
Macknight, RC
机构
[1] Univ Otago, Dept Biochem, Dunedin, New Zealand
[2] Univ Zurich, Inst Plant Biol, CH-8008 Zurich, Switzerland
[3] Univ Zurich, Zurich Basel Plant Sci Ctr, CH-8008 Zurich, Switzerland
关键词
D O I
10.1016/j.tplants.2005.06.006
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Laser-assisted microdissection (LAM) is a powerful tool for isolating specific tissues, cell types and even organelles from sectioned biological specimen in a manner conducive to the extraction of RNA, DNA or protein. LAM, which is an established technique in many areas of biology, has now been successfully adapted for use with plant tissues. Here, we provide an overview of the processes involved in conducting a successful LAM study in plants and review recent developments that have made this technique even more desirable. We also discuss how the technology might be exploited to answer some pertinent questions in plant biology.
引用
收藏
页码:397 / 406
页数:10
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