Laser capture microdissection of cells from plant tissues

被引:226
|
作者
Kerk, NM [1 ]
Ceserani, T [1 ]
Tausta, SL [1 ]
Sussex, IM [1 ]
Nelson, TM [1 ]
机构
[1] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
关键词
D O I
10.1104/pp.102.018127
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Laser capture microdissection (LCM) is a technique by which individual cells can be harvested from tissue sections while they are viewed under the microscope, by tacking selected cells to an adhesive film with a laser beam. Harvested cells can provide DNA, RNA, and protein for the profiling of genomic characteristics, gene expression, and protein spectra from individual cell types. We have optimized LCM for a variety of plant tissues and species, permitting the harvesting of cells from paraffin sections that maintain histological detail. We show that RNA can be extracted from LCM-harvested plant cells in amount and quality that are sufficient for the comparison of RNAs among individual cell types. The linear amplification of LCM-captured RNA should permit the expression profiling of plant cell types.
引用
收藏
页码:27 / 35
页数:9
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