Nucleosome disassembly during human non-homologous end joining followed by concerted HIRA- and CAF-1-dependent reassembly

被引：57

作者：

Li, Xuan ^{[1
]}

Tyler, Jessica K. ^{[1
]}

机构：

[1] Weill Cornell Med, Dept Pathol & Lab Med, New York, NY USA

来源：

ELIFE | 2016年 / 5卷

基金：

美国国家卫生研究院;

关键词：

STRAND BREAK REPAIR; DNA-REPAIR; INO80; COMPLEX; CHROMATIN; H3.3; TRANSCRIPTION; RESECTION; PATHWAY; PHOSPHORYLATION; RECRUITMENT;

D O I：

10.7554/eLife.15129

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cell achieves DNA double-strand break (DSB) repair in the context of chromatin structure. However, the mechanisms used to expose DSBs to the repair machinery and to restore the chromatin organization after repair remain elusive. Here we show that induction of a DSB in human cells causes local nucleosome disassembly, apparently independently from DNA end resection. This efficient removal of histone H3 from the genome during non-homologous end joining was promoted by both ATM and the ATP-dependent nucleosome remodeler IN080. Chromatin reassembly during DSB repair was dependent on the HIRA histone chaperone that is specific to the replication-independent histone variant H3.3 and on CAF-1 that is specific to the replication-dependent canonical histones H3.1/H3.2. Our data suggest that the epigenetic information is re-established after DSB repair by the concerted and interdependent action of replication -independent and replication-dependent chromatin assembly pathways.

引用

页数：18

共 35 条

[1] Transcription Recovery after DNA Damage Requires Chromatin Priming by the H3.3 Histone Chaperone HIRA [J].

Adam, Salome ;

Polo, Sophie E. ;

Almouzni, Genevieve .

CELL, 2013, 155 (01) :94-106

[2] Roles of ATM and NBS1 in chromatin structure modulation and DNA double-strand break repair [J].

Berkovich, Elijahu ;

Monnat, Raymond J., Jr. ;

Kastan, Michael B. .

NATURE CELL BIOLOGY, 2007, 9 (06) :683-U137

[3] The CAF-1 and Hir Histone Chaperones Associate with Sites of Meiotic Double-Strand Breaks in Budding Yeast [J].

Brachet, Elsa ;

Beneut, Claire ;

Serrentino, Maria-Elisabetta ;

Borde, Valerie .

PLOS ONE, 2015, 10 (05)

[4] Repair Pathway Choices and Consequences at the Double-Strand Break [J].

Ceccaldi, Raphael ;

Rondinelli, Beatrice ;

D'Andrea, Alan D. .

TRENDS IN CELL BIOLOGY, 2016, 26 (01) :52-64

[5] Acetylated lysine 56 on histone H3 drives chromatin assembly after repair and signals for the completion of repair [J].

Chen, Chin-Chuan ;

Carson, Joshua J. ;

Feser, Jason ;

Tamburini, Beth ;

Zabaronick, Susan ;

Linger, Jeffrey ;

Tyler, Jessica K. .

CELL, 2008, 134 (02) :231-243

[6] The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends [J].

Chen, Xuefeng ;

Cui, Dandan ;

Papusha, Alma ;

Zhang, Xiaotian ;

Chu, Chia-Dwo ;

Tang, Jiangwu ;

Chen, Kaifu ;

Pan, Xuewen ;

Ira, Grzegorz .

NATURE, 2012, 489 (7417) :576-+

[7] The yeast Fun30 and human SMARCAD1 chromatin remodellers promote DNA end resection [J].

Costelloe, Thomas ;

Louge, Raphael ;

Tomimatsu, Nozomi ;

Mukherjee, Bipasha ;

Martini, Emmanuelle ;

Khadaroo, Basheer ;

Dubois, Kenny ;

Wiegant, Wouter W. ;

Thierry, Agnes ;

Burma, Sandeep ;

van Attikum, Haico ;

Llorente, Bertrand .

NATURE, 2012, 489 (7417) :581-+

[8] Epigenetic regulation of genomic integrity [J].

Deem, Angela K. ;

Li, Xuan ;

Tyler, Jessica K. .

CHROMOSOMA, 2012, 121 (02) :131-151

[9] Chromatin assembly coupled to DNA repair: A new role for chromatin assembly factor I [J].

Gaillard, PHL ;

Martini, EMD ;

Kaufman, PD ;

Stillman, B ;

Moustacchi, E ;

Almouzni, G .

CELL, 1996, 86 (06) :887-896

[10] Nucleolin mediates nucleosome disruption critical for DNA double-strand break repair [J].

Goldstein, Michael ;

Derheimer, Frederick A. ;

Tait-Mulder, Jacqueline ;

Kastan, Michael B. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (42) :16874-16879

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