Time- and Oil-Dependent Transcriptomic and Physiological Responses to Deepwater Horizon Oil in Mahi-Mahi (Coryphaena hippurus) Embryos and Larvae

被引：121

作者：

Xu, Elvis Genbo ^{[1
]}

Mager, Edward M. ^{[2
]}

Grosell, Martin ^{[2
]}

Pasparakis, Christina ^{[2
]}

Schlenker, Lela S. ^{[2
]}

Stieglitz, John D. ^{[2
]}

Benetti, Daniel ^{[2
]}

Hazard, E. Starr ^{[3
,4
]}

Courtney, Sean M. ^{[3
]}

Diamante, Graciel ^{[1
]}

Freitas, Juliane ^{[1
]}

Hardiman, Gary ^{[3
,5
,6
]}

Schlenk, Daniel ^{[1
]}

机构：

[1] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA

[2] Univ Miami, Dept Marine Biol & Ecol, Miami, FL 33149 USA

[3] Med Univ South Carolina, Ctr Genom Med, Charleston, SC 29403 USA

[4] Med Univ South Carolina, Computat Biol Resource Ctr, Charleston, SC 29403 USA

[5] Med Univ South Carolina, Dept Med, Charleston, SC 29403 USA

[6] Med Univ South Carolina, Dept Publ Hlth Sci, Charleston, SC 29403 USA

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2016年 / 50卷 / 14期

关键词：

CRUDE-OIL; ZEBRAFISH EMBRYOS; TOXICITY; EXPOSURE; ACTIVATION; MECHANISMS; EXPRESSION; IMPAIRS; ALPHA;

D O I：

10.1021/acs.est.6b02205

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The Deepwater Horizon (DWH) oil spill contaminated the spawning habitats for numerous commercially and ecologically important fishes. Exposure to the water accommodated fraction (WAF) of oil from the spill has been shown to cause cardiac toxicity during early developmental stages across fishes. To better understand the molecular events and explore new pathways responsible for toxicity, RNA sequencing was performed in conjunction with physiological and morphological assessments to analyze the time-course (24, 48, and 96 h post fertilization (hpf)) of transcriptional and developmental responses in embryos/larvae of mahi-mahi exposed to WAF of weathered (slick) and source DWH oils. Slick oil exposure induced more pronounced changes in gene expression over time than source oil exposure. Predominant transcriptomic responses included alteration of EIF2 signaling, steroid biosynthesis, ribosome biogenesis cytochrome P450 pathway. At 96 hpf, slick oil exposure resulted in significant perturbations in eye development and peripheral nervous system, suggesting novel targets in addition to the heart may be involved in the developmental, toxicity of DHW oil. Comparisons of changes of cardiac genes with phenotypic responses were consistent with reduced heart rate and increased pericardial edema in larvae exposed to slick oil but not source oil.

引用

页码：7842 / 7851

页数：10

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