A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues

被引：16

作者：

Higuchi, Akon ^{[1
,2
,3
,4
]}

Wang, Ching-Tang ^{[1
]}

Ling, Qing-Dong ^{[5
,6
]}

Lee, Henry Hsin-Chung ^{[7
]}

Kumar, S. Suresh ^{[8
]}

Chang, Yung ^{[9
]}

Alarfaj, Abdullah A. ^{[2
]}

Munusamy, Murugan A. ^{[2
]}

Hsu, Shih-Tien ^{[10
]}

Wu, Gwo-Jang ^{[11
,12
]}

Umezawa, Akihiko ^{[3
]}

机构：

[1] Natl Cent Univ, Dept Chem & Mat Engn, Taoyuan, Taiwan

[2] King Saud Univ, Dept Bot & Microbiol, Riyadh, Saudi Arabia

[3] Natl Res Inst Child Hlth & Dev, Dept Reprod, Tokyo, Japan

[4] RIKEN, Nano Med Engn Lab, Wako, Saitama, Japan

[5] Natl Cent Univ, Inst Syst Biol & Bioinformat, Taoyuan, Taiwan

[6] Cathay Gen Hosp, Cathay Med Res Inst, Taipei, Taiwan

[7] Cathay Gen Hosp, Dept Surg, Taipei, Taiwan

[8] Univ Putra Malaysia, Dept Med Microbiol & Parasitol, Slangor, Malaysia

[9] Chung Yuan Christian Univ, R&D Ctr Membrane Technol, Dept Chem Engn, Taoyuan, Taiwan

[10] Taiwan Landseed Hosp, Dept Internal Med, Taoyuan, Taiwan

[11] Tri Serv Gen Hosp, Natl Def Med Ctr, Grad Inst Med Sci, Taipei, Taiwan

[12] Tri Serv Gen Hosp, Natl Def Med Ctr, Dept Obstet & Gynecol, Taipei, Taiwan

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

FEEDER-FREE; DIFFERENTIATION; FIBROBLASTS; INDUCTION; SCAFFOLDS; DESIGN;

D O I：

10.1038/srep10217

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size from 8 to 25 mu m, and the membranes were incubated in cell culture medium for 15-18 days. The hADSCs that migrated from the membranes contained an extremely high percentage (e.g., >98%) of cells positive for mesenchymal stem cell markers and showed almost one order of magnitude higher expression of some pluripotency genes (Oct4, Sox2, Klf4 and Nanog) compared with cells isolated using the conventional culture method.

引用

页数：11

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