Ty3/Gypsy retrotransposons in the Pacific abalone Haliotis discus hannai: characterization and use for species identification in the genus Haliotis

被引:3
|
作者
Lee, Sung-Il [1 ]
Gim, Jeong-An [2 ,3 ,4 ]
Lim, Min-Ji [5 ]
Kim, Heui-Soo [2 ,3 ]
Nam, Bo-Hye [6 ]
Kim, Nam-Soo [5 ,7 ]
机构
[1] Korea Atom Energy Res Inst, Adv Radiat Technol Inst, 1266 Sinjeong, Jeongeup 580185, Jeonbuk, South Korea
[2] Pusan Natl Univ, Dept Biol Sci, Coll Nat Sci, Busan 46241, South Korea
[3] Pusan Natl Univ, Genet Engn Inst, Busan 46241, South Korea
[4] UNIST, Dept Life Sci, Genom Inst, Ulsan 44919, South Korea
[5] Kangwon Natl Univ, Inst Biotechnol & Biosci, Chunchon 24341, South Korea
[6] Natl Inst Fisheries Sci, Biotechnol Res Div, Busan 46083, South Korea
[7] Kangwon Natl Univ, Dept Mol Biosci, Chunchon 24341, South Korea
关键词
Haliotis species; Abalone; Ty3/Gypsy retrotransposon; SSAP; SCAR; TRANSPOSABLE ELEMENTS; GENOME SIZE; ENDOGENOUS RETROVIRUS; EVOLUTION; RICE;
D O I
10.1007/s13258-017-0619-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transposable elements are highly abundant elements that are present in all eukaryotic species. Here, we present a molecular description of abalone retrotransposon (Abret) elements. The genome of Haliotis discus hannai contains 130 Abret elements which were all Ty3/Gypsy retrotransposons. The Ty1/Copia elements were absent in the H. discus hannai genome. Most of the elements were not complete due to sequence truncation or coding region decay. However, three elements Abret-296, Abret-935, and Abret-3259 had most of the canonical features of LTR (long terminal repeat)-retrotransposons. There were several reading frame shifts in Abret-935 and Abret-3259 elements. Surprisingly, phylogenetic analysis indicated that all of the elements belonged to the Osvaldo lineage. The sequence divergence between LTRs revealed that the Abret elements were mostly active within 2 million years ago. Abret elements were used as molecular markers in SSAP analyses, which allowed clear distinction of different species in the genus Haliotis. The polymorphic markers were converted into SCAR markers for use in species identification by simple PCR in the Haliotis genus.
引用
收藏
页码:177 / 187
页数:11
相关论文
共 50 条
  • [21] Carbonic Anhydrase in Pacific Abalone Haliotis discus hannai: Characterization, Expression, and Role in Biomineralization
    Sharker, Md. Rajib
    Kim, Soo Cheol
    Hossen, Shaharior
    Sumi, Kanij Rukshana
    Choi, Sang Ki
    Choi, Kap Seong
    Kho, Kang Hee
    FRONTIERS IN MOLECULAR BIOSCIENCES, 2021, 8
  • [22] Repeatability of physiological traits in juvenile Pacific abalone, Haliotis discus hannai
    Gabriel G. González
    Katherina B. Brokordt
    Federico E. Winkler
    Marine Biology, 2010, 157 : 2195 - 2203
  • [23] An AFLP genetic linkage map of pacific abalone (Haliotis discus hannai)
    Qi L.
    Yanhong X.
    Ruihai Y.
    Akihiro K.
    Journal of Ocean University of China, 2007, 6 (3) : 259 - 267
  • [24] Identification and dimorphic expression of sex-related genes in Pacific abalone (Haliotis discus hannai)
    Wu, Yiyu
    Peng, Wenzhu
    Wang, Yi
    Huang, Zekun
    Feng, Yu
    Han, Zhaofang
    Luo, Xuan
    You, Weiwei
    Ke, Caihuan
    AQUACULTURE, 2023, 574
  • [25] Identification, characterization, and expression analysis of a serotonin receptor involved in the reproductive process of the Pacific abalone, Haliotis discus hannai
    Md. Rajib Sharker
    Zahid Parvez Sukhan
    Soo Cheol Kim
    Won Kyo Lee
    Kang Hee Kho
    Molecular Biology Reports, 2020, 47 : 555 - 567
  • [26] Mapping and marker identification for sex-determining in the Pacific abalone, Haliotis discus hannai Ino
    Luo, Hanjiao
    Xiao, Junzhu
    Jiang, Yonghua
    Ke, Yizhou
    Ke, Caihuan
    Cai, Mingyi
    AQUACULTURE, 2021, 530
  • [27] Identification and characteristics of muscle growth-related microRNA in the Pacific abalone, Haliotis discus hannai
    Jianfang Huang
    Xuan Luo
    Miaoqin Huang
    Guangmou Liu
    Weiwei You
    Caihuan Ke
    BMC Genomics, 19
  • [28] Identification, characterization, and expression analysis of a serotonin receptor involved in the reproductive process of the Pacific abalone, Haliotis discus hannai
    Sharker, Md Rajib
    Sukhan, Zahid Parvez
    Kim, Soo Cheol
    Lee, Won Kyo
    Kho, Kang Hee
    MOLECULAR BIOLOGY REPORTS, 2020, 47 (01) : 555 - 567
  • [29] Metabolomics Adaptation of Juvenile Pacific Abalone Haliotis discus hannai to Heat Stress
    Xu, Fei
    Gao, Tingting
    Liu, Xiao
    SCIENTIFIC REPORTS, 2020, 10 (01)
  • [30] Development of 127 Novel Microsatellite Markers for the Pacific Abalone, Haliotis discus hannai
    Zhang, Zhen
    Zhan, Aibin
    Hu, Jingjie
    Bao, Zhenmin
    Lu, Chuanqi
    Li, Weiwei
    Tian, Le
    Liu, Xiao
    JOURNAL OF THE WORLD AQUACULTURE SOCIETY, 2011, 42 (03) : 423 - 434