Inhibition of apoptosis by a Bombyx mori gene

被引：50
作者：
Kim, EJ
Rhee, WJ
Park, TH
机构：
[1] Seoul Natl Univ, Sch Chem Engn, Seoul 151744, South Korea
[2] Seoul Natl Univ, Inst Chem Proc, Seoul 151744, South Korea
来源：
BIOTECHNOLOGY PROGRESS | 2004年 / 20卷 / 01期
关键词：
D O I：
10.1021/bp034130y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An apoptosis-inhibiting component of silkworm hemolymph, isolated and characterized in our previous study, showed 95% N-terminal amino acid sequence homology with one of the 30K proteins, a group of structurally related proteins. The 30K protein was expressed in mammalian HEK293 cells and CHOK1 cells by transfection with 30Kc6. The expression of 30Kc6 inhibited apoptosis comparably to that of whole silkworm hemolymph, indicating that both intracellular expression and external supplementation inhibited apoptosis. The expression of 30Kc6 resulted in lower intracellular activity for caspase 3. However, the results of in vitro assay of caspase 3 show that the 30Kc6 protein does not inhibit caspase 3 activity. This indicates that the 30Kc6 protein inhibits the apoptosis by working in a further upstream event than caspase 3 activation.
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页码：324 / 329
页数：6
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