Transfection with different connexin genes alters growth and differentiation of human choriocarcinoma cells

被引:49
作者
Hellmann, P
Grümmer, R
Schirrmacher, K
Rook, M
Traub, O
Winterhager, E
机构
[1] Univ Essen Gesamthsch, Sch Med, Inst Anat, D-45122 Essen, Germany
[2] Univ Essen Gesamthsch, Sch Med, Inst Physiol, D-45122 Essen, Germany
[3] Univ Utrecht, Dept Med Physiol & Sports Med, NL-3508 TA Utrecht, Netherlands
[4] Univ Bonn, Inst Genet, Dept Mol Genet, D-53117 Bonn, Germany
关键词
gap junctions; malignant trophoblast; beta-hCG; electrophysiology;
D O I
10.1006/excr.1998.4332
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
To examine the role of cell-cell communication via gap junctions in controlling proliferation and differentiation we transfected the malignant trophoblast cell line Jeg-3, which exhibits extremely low cell-cell communication mediated by endogenously expressed connexin40, with connexin26, connexin40, and connexin43, respectively. In vitro growth of all cell clones transfected with connexin genes was significantly reduced compared to controls. This effect corresponded to a significant increase in total junctional conductance of all clones. Single-channel conductances for channels formed by the transfected connexins were in the range of the values published previously. Though total junctional conductance varied highly among clones and even within one clone, differentiation of the cells indicated by beta-hCG secretion was most prominent in the clones that revealed the largest amount of well-coupled cell pairs. Connexin26 channels enable cells of one clone to reduce drastically growth rate and produce significantly higher secretion of beta-hCG. Connexin43 had only moderate effects on the differentiation properties of Jeg-3 cells. These findings suggest that restoration of cell-cell communication plays a role in growth reduction and in differentiation of tumor cells and that different channel proteins might have different effects, (C) 1999 Academic Press.
引用
收藏
页码:480 / 490
页数:11
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