Constituents of Brazilian red propolis and their preferential cytotoxic activity against human pancreatic PANC-1 cancer cell line in nutrient-deprived condition

被引：122

作者：

Awale, Suresh ^{[1
]}

Li, Feng ^{[1
]}

Onozuka, Hiroko ^{[2
]}

Esurni, Hiroyasu ^{[2
]}

Tezuka, Yasuhiro ^{[1
]}

Kadota, Shigetoshi ^{[1
]}

机构：

[1] Toyama Univ, Ins Nat Med, Toyama 9300194, Japan

[2] Natl Canc Ctr Res Inst E, Invest Treatment Div, Chiba 2778577, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2008年 / 16卷 / 01期

关键词：

D O I：

10.1016/j.bmc.2007.10.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human pancreatic cancer cells such as PANC-1 are known to exhibit marked tolerance to nutrition starvation that enables them to survive for prolonged period of time even under extremely nutrient-deprived conditions. Thus, elimination of this tolerance to nutrition starvation is regarded as a novel approach in anticancer drug development. In this study, the MeOH soluble extract of Brazilian red propolis was found to kill 100%, PANC-1 cells preferentially in the nutrient-deprived condition at the concentration of 10 mu g/mL. Further phytochemical investigation led to the isolation of 43 compounds including three new compounds, (6aS,11aS)-6a-ethoxymedicarpan (1), 2-(2',4'-dihydroxyphenyl)-3-methyl-6-methoxybenzofuran (2), and 2,6-dihydroxy-2-[(4-hydroxyphenyl)methyl]-3-benzofuranone (3). Among them, (6aR,11aR)-3,8-dihydroxy-9-methoxypterocarpan (21, DMPC) displayed the most potent 100% preferential cytotoxicity (PC100) at the concentration of 12.5 mu M. Further study on the mode of cell death induced by DMPC against PANC-1 cells indicated that killing process was not accompanied by DNA fragmentation, rather through a nonapoptotic pathway accompanied by necrotic-type morphological changes. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：181 / 189

页数：9

共 47 条

[1] CHEMICAL CONSTITUENTS OF ALNUS-SIEBOLDIANA (BETULACEAE) .2. ISOLATION AND STRUCTURE OF FLAVONOIDS AND STILBENES [J].