Phosphorous deficiency decreases nitrogenase activity but increases proton efflux in N2-fixing Medicago truncatula

被引:6
作者
Schulze, J. [2 ]
Mohamed, M. A. N. [3 ]
Carlsson, G. [1 ,4 ]
Drevon, J. J. [1 ]
机构
[1] INRA, UMR 1222, F-34060 Montpellier 2, France
[2] Univ Gottingen, Dept Nutzpflanzenwissensch, Abt Pflanzenernahrung, D-37075 Gottingen, Germany
[3] Suez Canal Univ, Dept Soil & Water, N Sinai, Egypt
[4] Dept Agr Farming Syst Technol & Prod Qual, SE-23053 Alnarp, Sweden
关键词
Model legume; Nitrogen fixation; P deficiency; Sinorhizobium meliloti; Symbiosis; SYMBIOTIC N-2 FIXATION; INDUCED DECLINE; NODULES; RELEASE;
D O I
10.1016/j.plaphy.2011.01.022
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Effects of Sinorhizobium strain and P nutrition on N-2-dependent growth, nitrogenase activity and proton efflux by nodulated roots were investigated in the model legume Medicago truncatula cultivar Jemalong grown in hydroaeroponic culture in symbioses with Sinorhizobium meliloti strains 102F51 and 2011. Sinorhizobium strain had strong effects on nitrogenase activity and N-2-dependent growth, with S. meliloti 102F51 being the more efficient strain. Apparent and total nitrogenase activities, measured by hydrogen evolution in air and argon, respectively, were drastically reduced in plants supplied with 5 mu mol P plant(-1) week(-1) as compared with 15 mu mol P plant(-1) week(-1). There was a net proton efflux as soon as 2 weeks after inoculation and, in contrast to the effect of P nutrition on nitrogenase activity, P deficiency increased total and specific proton effluxes, irrespective of Sinorhizobium strain. (C) 2011 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:458 / 460
页数:3
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