Extraction Separation of BSA in Aqueous Two-Phase Systems of Anionic and Cationic Surfactant Mixtures

被引：12

作者：

Teng, Hongni ^{[1
]}

Li, Ning ^{[1
]}

Zhu, Xixi ^{[1
]}

Chen, Yong ^{[1
]}

机构：

[1] Shandong Univ Sci & Technol, Coll Chem & Environm Engn, Dept Appl Chem, Qingdao 266510, Peoples R China

来源：

JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY | 2011年 / 32卷 / 06期

关键词：

Anionic and cationic surfactants; aqueous two-phase system; extraction; CETYLTRIMETHYLAMMONIUM BROMIDE; PHASE-BEHAVIOR; POLYMER; MICELLES; PROTEINS; PAIR;

D O I：

10.1080/01932691.2010.488141

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Aqueous two-phase systems (ATPS) are formed in mixtures of sodium dodecylsulfate (SDS), cetyltrimethyl ammonium bromide (CTAB), and sodium bromide (NaBr). Two different kinds of ATPS appear when CTAB and SDS surfactants are in excess, respectively. Such ATPS may provide a new, useful partitioning system for separation of bovine serum albumin (BSA). The partitioning of BSA was studied in these systems. The results show that the partitioning is affected by the electrical property, the hydrophobicity and the structure of liquid crystal (LC), which exists in the top phase of the ATPS. BSA is extracted into the top phase with higher distribution coefficient when LC and BSA are oppositely charged. The hydrophobicity of LC, which can be improved by increasing the length of alkyl group, enhances the distribution coefficient. The hydrophobicity of lamellar LC and hexagonal LC is stronger than that of cubic LC, which causes extractive capability of the former is higher than the latter.

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收藏

页码：828 / 833

页数：6

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