Identification of herbal medicinal materials using DNA barcodes

被引:92
作者
Li, Ming [1 ]
Cao, Hui [2 ]
But, Paul Pui-Hay [3 ]
Shaw, Pang-Chui [1 ,3 ]
机构
[1] Chinese Univ Hong Kong, State Key Lab Phytochem & Plant Resources W China, Inst Chinese Med, Shatin, Hong Kong, Peoples R China
[2] Natl Engn Res Ctr Modernizat Tradit Chinese Med, Zhuhai 519020, Guangdong, Peoples R China
[3] Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China
关键词
DNA barcode; herbal medicinal materials; internal transcribed spacer (ITS); matK; molecular identification; rbcL; trnH-psbA; RIBOSOMAL-RNA GENE; PHYLOGENETIC RELATIONSHIP; MOLECULAR AUTHENTICATION; MATK GENE; SEQUENCE-ANALYSIS; CHLOROPLAST MATK; RBCL SEQUENCES; TRNL-F; SPACER; PLANTS;
D O I
10.1111/j.1759-6831.2011.00132.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Herbal medicinal materials have been used worldwide for centuries to maintain health and to treat disease. However, adulteration of herbal medicines remains a major concern of users and industry for reasons of safety and efficacy. identification of herbal medicinal materials by DNA technology has been widely applied, started from the mid-1990s. In recent years, DNA barcoding of global plant species using four standard barcodes (rbcL, matK, trnH-psbA and ITS) has been a major focus in the fields of biodiversity and conservation. These DNA barcodes can also be used as reliable tools to facilitate the identification of herbal medicinal materials for the safe use of herbs, quality control, and forensic investigation. Many studies have applied these DNA barcodes for the identification of herbal medicinal species and their adulterants. The present article reviews efforts in the identification of herbal medicinal materials using the standard DNA barcodes and other DNA sequence-based markers.
引用
收藏
页码:271 / 283
页数:13
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