Nitrogen removal efficiency and microbial community analysis of ANAMMOX biofilter at ambient temperature

被引：18
作者：
Zeng Taotao [1 ]
Li Dong [2 ]
Zeng Huiping [2 ]
Xie Shuibo [1 ]
Qiu Wenxin [3 ]
Liu Yingjiu [1 ]
Zhang Jie [2 ,4 ]
机构：
[1] Univ South China, Hunan Prov Key Lab Pollut Control & Resources Reu, Hengyang 421001, Peoples R China
[2] Beijing Univ Technol, Key Lab Beijing Water Qual Sci & Water Environm R, Beijing 100124, Peoples R China
[3] Beijing Gen Municipal Engn Design & Res Inst Co L, Beijing 100082, Peoples R China
[4] Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China
来源：
WATER SCIENCE AND TECHNOLOGY | 2015年 / 71卷 / 05期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
ambient temperature; ANAMMOX; microbial community; nitrogen removal; WASTE-WATER; BACTERIA; DIVERSITY;
D O I：
10.2166/wst.2015.019
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
An upflow anaerobic biofilter (AF) was developed to investigate anaerobic ammonium-oxidizing (ANAMMOX) efficiency in treating low-strength wastewater at ambient temperature (15.3-23.2 degrees C). Denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization were used to investigate treatment effects on the microbial community. Stepwise decreases in influent ammonia concentration could help ANAMMOX bacteria selectively acclimate to low-ammonia conditions. With an influent ammonia concentration of 46.5 mg/L, the AF reactor obtained an average nitrogen removal rate of 2.26 kg/(m(3) day), and a removal efficiency of 75.9%. polymerase chain reaction-DGGE results showed that microbial diversity in the low matrix was greater than in the high matrix. Microbial community structures changed when the influent ammonia concentration decreased. The genus of functional ANAMMOX bacteria was Candidatus Kuenenia stuttgartiensis, which remained stationary across study phases. Visual observation revealed that the relative proportions of ANAMMOX bacteria decreased from 41.6 to 36.3% across three study phases. The AF bioreactor successfully maintained high activity due to the ANAMMOX bacteria adaptation to low temperature and substrate conditions.
引用
收藏
页码：725 / 733
页数：9
相关论文
共 18 条
[1]   PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].
AMANN, RI ;
LUDWIG, W ;
SCHLEIFER, KH .
MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169
[2]  
[Anonymous], 2006, STANDARD METHODS EXA, DOI DOI 10.5860/CHOICE.37-2792
[3]   Silver staining DNA in polyacrylamide gels [J].
Bassam, Brant J. ;
Gresshoff, Peter M. .
NATURE PROTOCOLS, 2007, 2 (11) :2649-2654
[4]  
Chen Jun, 2008, Huanjing Kexue, V29, P1092
[5]   OLAND is feasible to treat sewage-like nitrogen concentrations at low hydraulic residence times [J].
De Clippeleir, Haydee ;
Yan, Xungang ;
Verstraete, Willy ;
Vlaeminck, Siegfried Elias .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2011, 90 (04) :1537-1545
[6]   Short- and long-term effects of temperature on the Anammox process [J].
Dosta, J. ;
Fernandez, I. ;
Vazquez-Padin, J. R. ;
Mosquera-Corral, A. ;
Campos, J. L. ;
Mata-Alvarez, J. ;
Mendez, R. .
JOURNAL OF HAZARDOUS MATERIALS, 2008, 154 (1-3) :688-693
[7]   Autotrophic nitrogen removal from low strength waste water at low temperature [J].
Hendrickx, Tim L. G. ;
Wang, Yang ;
Kampman, Christel ;
Zeeman, Grietje ;
Temmink, Hardy ;
Buisman, Cees J. N. .
WATER RESEARCH, 2012, 46 (07) :2187-2193
[8]   Nitrogen removal performance using anaerobic ammonium oxidation at low temperatures [J].
Isaka, Kazuichi ;
Date, Yasuhiro ;
Kimura, Yuya ;
Sumino, Tatsuo ;
Tsuneda, Satoshi .
FEMS MICROBIOLOGY LETTERS, 2008, 282 (01) :32-38
[9]   Sewage Treatment with Anammox [J].
Kartal, B. ;
Kuenen, J. G. ;
van Loosdrecht, M. C. M. .
SCIENCE, 2010, 328 (5979) :702-703
[10]   Distribution and genetic diversity of functional microorganisms in different CANON reactors [J].
Liu, Tao ;
Li, Dong ;
Zeng, Huiping ;
Li, Xiangkun ;
Liang, Yuhai ;
Chang, Xiaoyan ;
Zhang, Jie .
BIORESOURCE TECHNOLOGY, 2012, 123 :574-580
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