MYC, TP53, and Chromosome 17 Copy-Number Alterations in Multiple Gastric Cancer Cell Lines and in Their Parental Primary Tumors

被引：36

作者：

Leal, Mariana Ferreira ^{[1
]}

Calcagno, Danielle Queiroz ^{[2
]}

Ferreira Borges da Costa, Joana de Fatima ^{[2
]}

Raiol Silva, Tanielly Cristina ^{[2
]}

Khayat, Andre Salim ^{[2
]}

Chen, Elizabeth Suchi ^{[1
]}

Assumpcao, Paulo Pimentel ^{[3
]}

Cardoso Smith, Marilia de Arruda ^{[1
]}

Burbano, Rommel Rodriguez ^{[2
]}

机构：

[1] Univ Fed Sao Paulo, Dept Morphol & Genet, Div Genet, BR-04023900 Sao Paulo, Brazil

[2] Fed Univ Para, Inst Biol Sci, Human Cytogenet Lab, BR-66073000 Belem, Para, Brazil

[3] Fed Univ Para, Joao de Barros Barreto Univ Hosp, Surg Serv, BR-60673000 Belem, Para, Brazil

来源：

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2011年

基金：

巴西圣保罗研究基金会;

关键词：

IN-SITU HYBRIDIZATION; CONVENTIONAL CYTOGENETIC CHARACTERIZATION; NORTHERN BRAZIL; C-MYC; AMPLIFICATION; INDIVIDUALS; ANEUPLOIDY; IMMORTALIZATION; ABERRATIONS; INSTABILITY;

D O I：

10.1155/2011/631268

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We evaluated whether MYC, TP53, and chromosome 17 copy-number alterations occur in ACP02, ACP03, and AGP01 gastric cancer cell lines and in their tumor counterpart. Fluorescence in situ hybridization for MYC and TP53 genes and for chromosome 17 was applied in the 6th, 12th, 60th, and 85th passages of the cell lines and in their parental primary tumors. We observed that three and four MYC signals were the most common alterations in gastric cell lines and tumors. ACP02 presented cells with two copies of chr17 and loss of one copy of TP53 more frequently than ACP03 and AGP01. Only ACP03 and AGP01 presented clonal chr17 trisomy with three or two TP53 copies. The frequency of MYC gain, TP53 loss, and chromosome 17 trisomy seems to increase in gastric cell lines compared to their parental tumors. Our findings reveal that these cell lines retain, in vitro, the genetic alterations presented in their parental primary tumors.

引用

页数：8

共 32 条

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