Real-Time PCR Assay for the Identification of the Brown Marmorated Stink Bug (Halyomorpha halys)

被引：17

作者：

Dhami, Manpreet K. ^{[1
,2
]}

Dsouza, Melissa ^{[3
]}

Waite, David W. ^{[1
]}

Anderson, Diane ^{[1
]}

Li, Dongmei ^{[1
]}

机构：

[1] Minist Primary Ind, Plant Hlth & Environm Lab, Auckland, New Zealand

[2] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[3] Univ Chicago, Dept Ecol & Evolut, 940 E 57Th St, Chicago, IL 60637 USA

来源：

FRONTIERS IN MOLECULAR BIOSCIENCES | 2016年 / 3卷

关键词：

qPCR; invasive species; diagnostic test; Pentatomidae; biosecurity; HEMIPTERA-PENTATOMIDAE; SYBR GREEN; HETEROPTERA; STAL;

D O I：

10.3389/fmolb.2016.00005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), is a gregarious crop pest that has rapidly spread across the world in the last two decades. It is an excellent hitchhiker species, especially as an over-wintering adult. During this period it is often associated with non-biological commodities such as shipping containers and machinery that travel long distances. Inadequate identification keys and similarity to common species has assisted its spread across Europe, while accurate identification from immature stages or eggs is not possible. We developed a real-time TaqMan PCR assay for the accurate and sensitive detection of the brown marmorated stink bug from all life stages. The assay performance against required diagnostic criterion and within a quarantine framework are described.

引用

页数：6

共 35 条

[21] Seasonal Occurrence and Impact of Halyomorpha halys (Hemiptera: Pentatomidae) in Tree Fruit [J].

Nielsen, Anne L. ;

Hamilton, George C. .

JOURNAL OF ECONOMIC ENTOMOLOGY, 2009, 102 (03) :1133-1140

[22] Real-time RT-PCR (TaqMan®) assays for the detection of Grapevine Leafroll associated viruses 1-5 and 9 [J].

Osman, Fatima ;

Leutenegger, Christian ;

Golino, Deborah ;

Rowhani, Adib .

JOURNAL OF VIROLOGICAL METHODS, 2007, 141 (01) :22-29

[23]

Pournelle G. H., 1953, Journal of Mammalogy, V34, P133, DOI 10.1890/0012-9658(2002)083[1421:SDEOLC]2.0.CO

[24]

[25] BOLD: The Barcode of Life Data System (www.barcodinglife.org) [J].

Ratnasingham, Sujeevan ;

Hebert, Paul D. N. .

MOLECULAR ECOLOGY NOTES, 2007, 7 (03) :355-364

[26] qpcR:: an R package for sigmoidal model selection in quantitative real-time polymerase chain reaction analysis [J].

Ritz, Christian ;

Spiess, Andrej-Nikolai .

BIOINFORMATICS, 2008, 24 (13) :1549-1551

[27]

Robertson I. A. D., 2009, PENTATOMOIDEA HEMIPT

[28] Insect Pest Problems of Sweet Persimmon in Korea [J].

Son, J. K. ;

Yun, Ji Eun ;

Park, C. G. .

IV INTERNATIONAL SYMPOSIUM ON PERSIMMON, 2009, 833 :325-330

[29] First record of the brown marmorated stink bug, Halyomorpha halys (Hemiptera: Heteroptera: Pentatomidae), in Hungary, with description of the genitalia of both sexes [J].

Vetek, Gabor ;

Papp, Veronika ;

Haltrich, Attila ;

Redei, David .

ZOOTAXA, 2014, 3780 (01) :194-200

[30] Development of a sequence-specific real-time PCR to the melon thrips Thrips palmi (Thysan., Thripidae) [J].

Walsh, K ;

Boonham, N ;

Barker, I ;

Collins, DW .

JOURNAL OF APPLIED ENTOMOLOGY, 2005, 129 (05) :272-279

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