Sensitive fluorescence sensor for point-of-care detection of trypsin using glutathione-stabilized gold nanoclusters

被引：45

作者：

Li, Hongxia ^{[1
]}

Yang, Mingming ^{[2
]}

Kong, Deshuai ^{[1
]}

Jin, Rui ^{[1
]}

Zhao, Xu ^{[1
]}

Liu, Fangmeng ^{[1
]}

Yan, Xu ^{[1
]}

Lin, Yuehe ^{[3
]}

Lu, Geyu ^{[1
]}

机构：

[1] Jilin Univ, Coll Elect Sci & Engn, State Key Lab Integrated Optoelect, 2699 Qianjin St, Changchun 130012, Jilin, Peoples R China

[2] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

[3] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2019年 / 282卷

基金：

中国博士后科学基金;

关键词：

Fluorescence; Gold nanoclusters; Electron transfer; Trypsin; Test strips; AGGREGATION-INDUCED EMISSION; GRAPHENE QUANTUM DOTS; BOVINE SERUM-ALBUMIN; LABEL-FREE; PROTEASE DETECTION; CYTOCHROME-C; QUANTIFICATION; NANOPARTICLES; BILIRUBIN; ENZYME;

D O I：

10.1016/j.snb.2018.11.077

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

There is an urgent demand for on-site detection of biomarker, particularly in clinical diagnosis and therapeutics applications. Herein, we designed a convenient and sensitive fluorescence nanosensor for point-of-care determination of trypsin (TRY). The fluorescence intensity of gold nanoclusters (AuNCs) can be quenched by cytochrome c (Cyt c) via electron transfer mechanism. TRY specifically catalyzes the hydrolysis of Cyt c to produce small peptide fragments, inducing the significant fluorescence recovery. As a result, the AuNCs-based system processed a sensitive and selective response to TRY with the range 0.2-100 mu g mL(-1), accompanying a detection limit of 0.08 mu g mL(-1). Significantly, the sensing assay can be used to construct test strips for rapid and visual recognition of TRY. Combining with smartphone and ImageJ software, we further developed an image processing algorithm for quantitative detection of TRY with highly promising, which validated the potential point-of-care application.

引用

页码：366 / 372

页数：7

共 38 条

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