A Multiplex Label-Free Approach to Avian Influenza Surveillance and Serology

被引:18
作者
Bucukovski, Joseph [1 ,2 ]
Latorre-Margalef, Neus [3 ]
Stallknecht, David E. [3 ]
Miller, Benjamin L. [1 ,2 ]
机构
[1] Univ Rochester, Dept Biomed Engn, Rochester, NY 14642 USA
[2] Univ Rochester, Dept Dermatol, Rochester, NY 14627 USA
[3] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Athens, GA USA
来源
PLOS ONE | 2015年 / 10卷 / 08期
基金
瑞典研究理事会;
关键词
ARRAYED IMAGING REFLECTOMETRY; PROTEIN MICROARRAY; VIRUS; RESPONSES; ANTIBODIES; INFECTION; VACCINE; ASSAYS;
D O I
10.1371/journal.pone.0134484
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
l;Influenza serology has traditionally relied on techniques such as hemagglutination inhibition, microneutralization, and ELISA. These assays are complex, challenging to implement in a format allowing detection of several types of antibody-analyte interactions at once (multiplex), and troublesome to implement in the field. As an alternative, we have developed a hemagglutinin microarray on the Arrayed Imaging Reflectometry (AIR) platform. AIR provides sensitive, rapid, and label-free multiplex detection of targets in complex analyte samples such as serum. In preliminary work, we demonstrated the application of this array to the testing of human samples from a vaccine trial. Here, we report the application of an expanded label-free hemagglutinin microarray to the analysis of avian serum samples. Samples from influenza virus challenge experiments in mallards yielded strong, selective detection of antibodies to the challenge antigen in most cases. Samples acquired in the field from mallards were also analyzed, and compared with viral hemagglutinin inhibition and microneutralization assays. We find that the AIR hemagglutinin microarray can provide a simple and robust alternative to standard methods, offering substantially greater information density from a simple workflow.
引用
收藏
页数:11
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