Genome-Wide Analysis of the LRR-RLP Gene Family in a Wild Banana (Musa acuminata ssp. malaccensis) Uncovers Multiple Fusarium Wilt Resistance Gene Candidates

被引:10
作者
Alvarez-Lopez, Dulce [1 ]
Aurora Herrera-Valencia, Virginia [1 ]
Gongora-Castillo, Elsa [2 ]
Garcia-Laynes, Sergio [1 ]
Puch-Hau, Carlos [3 ]
Alhucema Lopez-Ochoa, Luisa [4 ]
Lizama-Uc, Gabriel [5 ]
Peraza-Echeverria, Santy [1 ]
机构
[1] Ctr Invest Cient Yucatan, Unidad Biotecnol, Calle 43 130, Merida 97200, Yucatan, Mexico
[2] Ctr Invest Cient Yucatan, CONACYT Unidad Biotecnol, Calle 43 130, Merida 97200, Yucatan, Mexico
[3] Inst Politecn Nacl CINVESTAV IPN, Dept Recursos Mar, Unidad Merida, Ctr Invest & Estudios Avanzados, Km 6,Antigua Carretera Progreso, Merida 97310, Yucatan, Mexico
[4] Ctr Invest Cient Yucatan, Unidad Bioquim & Biol Mol Plantas, Calle 43 130, Merida 97200, Yucatan, Mexico
[5] Tecnol Nacl Mexico, Inst Tecnol Merida, Av Tecnol Km 4 5, Merida 97118, Yucatan, Mexico
关键词
banana; disease resistance; genome-wide analysis; LRR-RLP; Fusarium wilt; genetic improvement; RECEPTOR-LIKE PROTEINS; TOMATO; CLASSIFICATION; RECOGNITION; EVOLUTION; ROLES;
D O I
10.3390/genes13040638
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Banana is the most popular fruit in the world, with a relevant role in food security for more than 400 million people. However, fungal diseases cause substantial losses every year. A better understanding of the banana immune system should facilitate the development of new disease-resistant cultivars. In this study, we performed a genome-wide analysis of the leucine-rich repeat receptor-like protein (LRR-RLP) disease resistance gene family in a wild banana. We identified 78 LRR-RLP genes in the banana genome. Remarkably, seven MaLRR-RLPs formed a gene cluster in the distal part of chromosome 10, where resistance to Fusarium wilt caused by Foc race 1 has been previously mapped. Hence, we proposed these seven MaLRR-RLPs as resistance gene candidates (RGCs) for Fusarium wilt. We also identified seven other banana RGCs based on their close phylogenetic relationships with known LRR-RLP proteins. Moreover, phylogenetic analysis of the banana, rice, and Arabidopsis LRR-RLP families revealed five major phylogenetic clades shared by these plant species. Finally, transcriptomic analysis of the MaLRR-RLP gene family in plants treated with Foc race 1 or Foc TR4 showed the expression of several members of this family, and some of them were upregulated in response to these Foc races. Our study provides novel insights into the structure, distribution, evolution, and expression of the LRR-RLP gene family in bananas as well as valuable RGCs that will facilitate the identification of disease resistance genes for the genetic improvement of this crop.
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页数:21
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