Beneficial effects of whey protein peptides on muscle loss in aging mice models

被引:11
作者
Wu, Xin [1 ,2 ]
Yu, Xiaochen [1 ]
Zhu, Na [1 ]
Xu, Meihong [1 ]
Li, Yong [1 ]
机构
[1] Peking Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Beijing, Peoples R China
[2] Peking Univ, Peking Univ Res Ctr Aging, Sch Basic Med Sci, Dept Biochem & Mol Biol,Beijing Key Lab Protein Po, Beijing, Peoples R China
关键词
whey protein peptides; d-galactose (d-gal); sarcopenia; age-related; skeletal muscles; protein synthesis; muscle loss; C-REACTIVE PROTEIN; SKELETAL-MUSCLE; OLDER PERSONS; SARCOPENIA; INFLAMMATION; INGESTION; LEUCINE; RISK; MEN;
D O I
10.3389/fnut.2022.897821
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Aging-related muscle loss is a hallmark of aging and is the cause of some negative outcomes. An optimized diet and supplements have a positive effect in slowing down the process of muscle loss. D-galactose(d-gal) has been used widely to develop aging model. This study explored the beneficial effects of whey protein peptides (WPPs) on sarcopenia in d-gal-induced aging mice. A total of 72 SPF male C57BL/6N mice were used in this study. Sixty mice were modeled by injected intraperitoneally with d-gal (100 mg/kg body weight for 6 weeks), and the other 12 mice were used as control, and injected with the same amount of normal saline. After 6 weeks, the modeled mice were randomly divided into the model control group, whey protein group (1.5 g/kg*bw), and three WPPs intervention groups (0.3 g/kg*bw, 1.5 g/kg*bw, 3.0 g/kg*bw), according to serum malondialdehyde (MDA) level. The test samples were orally given to mice by daily garaged. During the 30 days intervention period, the model control group, whey protein group, and WPPs group continued receiving intraperitoneal injections of d-gal, whereas the control group continued receiving intraperitoneal injections of normal saline. The results showed that WPPs could significantly improve the grip strength of aged mice. WPPs could significantly increase lean mass of aged mice and increase muscle weight of gastrocnemius and extensor digitorum longus. WPPs could significantly increase the level of insulin-like growth factor-1 (IGF-1) and reduce level of interleukin (IL)-1, IL-6, tumor necrosis factor-alpha (TNF-alpha) in serum. WPPs could affect the muscle fiber size in d-gal-induced aging mice. Its specific mechanism may be related to the activation of IGF-1/Akt/mTOR protein synthesis signaling pathway and reduction of the level of inflammation. These results indicate that WPPs can improve aging-related sarcopenia. Compared with whey protein, WPPs supplement seems a better form for sarcopenia.
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页数:12
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共 47 条
[1]   Longitudinal evidence on the association between interleukin-6 and C-reactive protein with the loss of total appendicular skeletal muscle in free-living older men and women [J].
Aleman, Heliodoro ;
Esparza, Julian ;
Ramirez, Fatima Araceli ;
Astiazaran, Humberto ;
Payette, Helene .
AGE AND AGEING, 2011, 40 (04) :469-475
[2]   Chapter 4 Treatment of sarcopenia [J].
Arai, Hidenori ;
Wakabayashi, Hidetaka ;
Yoshimura, Yoshihiro ;
Yamada, Minoru ;
Kim, Hunkyung ;
Harada, Atsushi .
GERIATRICS & GERONTOLOGY INTERNATIONAL, 2018, 18 :28-44
[3]   D-Galactose-induced accelerated aging model: an overview [J].
Azman, Khairunnuur Fairuz ;
Zakaria, Rahimah .
BIOGERONTOLOGY, 2019, 20 (06) :763-782
[4]   Chronic low-grade inflammation and age-related sarcopenia [J].
Beyer, Ingo ;
Mets, Tony ;
Bautmans, Ivan .
CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE, 2012, 15 (01) :12-22
[5]   Soy-Dairy Protein Blend or Whey Protein Isolate Ingestion Induces Similar Postexercise Muscle Mechanistic Target of Rapamycin Complex 1 Signaling and Protein Synthesis Responses in Older Men [J].
Borack, Michael S. ;
Reidy, Paul T. ;
Husaini, Syed H. ;
Markofski, Melissa M. ;
Deer, Rachel R. ;
Richison, Abigail B. ;
Lambert, Bradley S. ;
Cope, Mark B. ;
Mukherjea, Ratna ;
Jennings, Kristofer ;
Volpi, Elena ;
Rasmussen, Blake B. .
JOURNAL OF NUTRITION, 2016, 146 (12) :2468-2475
[6]   Differential Responses of Blood Essential Amino Acid Levels Following Ingestion of High-Quality Plant-Based Protein Blends Compared to Whey Protein-A Double-Blind Randomized, Cross-Over, Clinical Trial [J].
Brennan, Jessica L. ;
Keerati-u-rai, Maneephan ;
Yin, Huaixia ;
Daoust, Julie ;
Nonnotte, Emilie ;
Quinquis, Laurent ;
St-Denis, Thierry ;
Bolster, Douglas R. .
NUTRIENTS, 2019, 11 (12)
[7]   Clinical Definitions of Sarcopenia and Risk of Hospitalization in Community-Dwelling Older Men: The Osteoporotic Fractures in Men Study [J].
Cawthon, Peggy M. ;
Lui, Li-Yung ;
Taylor, Brent C. ;
McCulloch, Charles E. ;
Cauley, Jane A. ;
Lapidus, Jodi ;
Orwoll, Eric ;
Ensrud, Kristine E. .
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 2017, 72 (10) :1383-1389
[8]   Anti-Apoptotic and Pro-Survival Effect of Alpinate Oxyphyllae Fructus (AOF) in a D-Galactose-Induced Aging Heart [J].
Chang, Yung-Ming ;
Chang, Hen-Hong ;
Kuo, Wei-Wen ;
Lin, Hung-Jen ;
Yeh, Yu-Lan ;
Viswanadha, Vijaya Padma ;
Tsai, Chin-Chuan ;
Chen, Ray-Jade ;
Chang, Hsin-Nung ;
Huang, Chih-Yang .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2016, 17 (04)
[9]   Contribution of specific diseases and injuries to changes in health adjusted life expectancy in 187 countries from 1990 to 2013: retrospective observational study [J].
Chen, He ;
Chen, Gong ;
Zheng, Xiaoying ;
Guo, Yan .
BMJ-BRITISH MEDICAL JOURNAL, 2019, 364
[10]   Asian Working Group for Sarcopenia: 2019 Consensus Update on Sarcopenia Diagnosis and Treatment [J].
Chen, Liang-Kung ;
Woo, Jean ;
Assantachai, Prasert ;
Auyeung, Tung-Wai ;
Chou, Ming-Yueh ;
Iijima, Katsuya ;
Jang, Hak Chul ;
Kang, Lin ;
Kim, Miji ;
Kim, Sunyoung ;
Kojima, Taro ;
Kuzuya, Masafumi ;
Lee, Jenny S. W. ;
Lee, Sang Yoon ;
Lee, Wei-Ju ;
Lee, Yunhwan ;
Liang, Chih-Kuang ;
Lim, Jae-Young ;
Lim, Wee Shiong ;
Peng, Li-Ning ;
Sugimoto, Ken ;
Tanaka, Tomoki ;
Won, Chang Won ;
Yamada, Minoru ;
Zhang, Teimei ;
Akishita, Masahiro ;
Arai, Hidenori .
JOURNAL OF THE AMERICAN MEDICAL DIRECTORS ASSOCIATION, 2020, 21 (03) :300-+