Mitotic inactivation of a human SWI/SNF chromatin remodeling complex

被引:230
作者
Sif, S
Stukenberg, PT
Kirschner, MW
Kingston, RE [1 ]
机构
[1] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
[2] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA
关键词
SWI/SNF complex; nucleosomes; phosphorylation; mitosis; chromatin;
D O I
10.1101/gad.12.18.2842
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
During mitosis, chromatin is condensed into mitotic chromosomes and transcription is inhibited, processes that might be opposed by the chromatin remodeling activity of the SWI/SNF complexes. Brg1 and hBrm, which are components of human SWI/SNF (hSWIISNF) complexes, were recently shown to be phosphorylated during mitosis. This suggested that phosphorylation might be used as a switch to modulate SWI/SNF activity. Using an epitope-tag strategy, we have purified hSWI/SNF complexes at different stages of the cell cycle, and found that hSWI/SNF was inactive in cells blocked in G(2)-M. Mitotic hSWI/SNF contained Brg1 but trot hBrm, and was phosphorylated on at least two subunits, hSWI3 and Brg1. In vitro, active hSWI/SNF from asynchronous cells can be phosphorylated and inactivated by ERK1, and reactivated by dephosphorylation. hSWI/SNF isolated as cells traversed mitosis regained activity when its subunits were dephosphorylated either in vitro or in vivo. We propose that this transitional inactivation and reactivation of hSWI/SNF is required for formation of a repressed chromatin structure during mitosis and reformation of an active chromatin structure as cells leave mitosis.
引用
收藏
页码:2842 / 2851
页数:10
相关论文
共 48 条
[1]   Signaling via mitogen-activated protein kinase kinase (MEK1) is required for Golgi fragmentation during mitosis [J].
Acharya, U ;
Mallabiabarrena, A ;
Acharya, JK ;
Malhotra, V .
CELL, 1998, 92 (02) :183-192
[2]  
Ausubel F.M., 1996, CURRENT PROTOCOLS MO
[3]   CELL-FREE SYSTEM FOR ASSEMBLY OF TRANSCRIPTIONALLY REPRESSED CHROMATIN FROM DROSOPHILA EMBRYOS [J].
BECKER, PB ;
WU, C .
MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (05) :2241-2249
[4]  
Bulger M., 1994, METH MOL G, V5, P241
[5]   RSC, an essential, abundant chromatin-remodeling complex [J].
Cairns, BR ;
Lorch, Y ;
Li, Y ;
Zhang, MC ;
Lacomis, L ;
ErdjumentBromage, H ;
Tempst, P ;
Du, J ;
Laurent, B ;
Kornberg, RD .
CELL, 1996, 87 (07) :1249-1260
[6]   A MULTISUBUNIT COMPLEX CONTAINING THE SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, AND SNF6 GENE-PRODUCTS ISOLATED FROM YEAST [J].
CAIRNS, BR ;
KIM, YJ ;
SAYRE, MH ;
LAURENT, BC ;
KORNBERG, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (05) :1950-1954
[7]   Sfh1p, a component of a novel chromatin-remodeling complex, is required for cell cycle progression [J].
Cao, YX ;
Cairns, BR ;
Kornberg, RD ;
Laurent, BC .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (06) :3323-3334
[8]   2 HUMAN HOMOLOGS OF SACCHAROMYCES-CEREVISIAE SW12/SNF2 AND DROSOPHILA-BRAHMA ARE TRANSCRIPTIONAL COACTIVATORS COOPERATING WITH THE ESTROGEN-RECEPTOR AND THE RETINOIC ACID RECEPTOR [J].
CHIBA, H ;
MURAMATSU, M ;
NOMOTO, A ;
KATO, H .
NUCLEIC ACIDS RESEARCH, 1994, 22 (10) :1815-1820
[9]   STIMULATION OF GAL4 DERIVATIVE BINDING TO NUCLEOSOMAL DNA BY THE YEAST SWI/SNF COMPLEX [J].
COTE, J ;
QUINN, J ;
WORKMAN, JL ;
PETERSON, CL .
SCIENCE, 1994, 265 (5168) :53-60
[10]   MOUSE ERK-1 GENE-PRODUCT IS A SERINE THREONINE PROTEIN-KINASE THAT HAS THE POTENTIAL TO PHOSPHORYLATE TYROSINE [J].
CREWS, CM ;
ALESSANDRINI, AA ;
ERIKSON, RL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (19) :8845-8849