DNA Interaction, DNA Photocleavage, Photocytotoxicity In Vitro, and Molecular Docking of Naphthyl-Appended Ruthenium Complexes

被引：12

作者：

Hu, Xia ^{[1
]}

Luo, Qian ^{[1
]}

Qin, Yao ^{[1
]}

Wu, Yao ^{[1
]}

Liu, Xue-Wen ^{[1
]}

机构：

[1] Hunan Univ Arts & Sci, Coll Chem & Mat Engn, Hunan Prov Engn Res Ctr Electroplating Wastewater, Hunan Prov Key Lab Water Treatment Funct Mat, Changde 415000, Peoples R China

来源：

MOLECULES | 2022年 / 27卷 / 12期

关键词：

ruthenium complex; DNA interaction; photocleavage; molecular docking; photocytotoxicity; ETHIDIUM-BROMIDE; SINGLET OXYGEN; BINDING; LIGAND; TRIS(PHENANTHROLINE)RUTHENIUM(II); PHOTOSENSITIZERS; EFFICIENT; STRATEGY;

D O I：

10.3390/molecules27123676

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

With the development of metal-based drugs, Ru(II) compounds present potential applications of PDT (photodynamic therapy) and anticancer reagents. We herein synthesized two naphthyl-appended ruthenium complexes by the combination of the ligand with naphthyl and bipyridyl. The DNA affinities, photocleavage abilities, and photocytotoxicity were studied by various spectral methods, viscosity measurement, theoretical computation method, gel electrophoresis, and MTT method. Two complexes exhibited strong interaction with calf thymus DNA by intercalation. Production of singlet oxygen (O-1(2)) led to obvious DNA photocleavage activities of two complexes under 365 nm light. Furthermore, two complexes displayed obvious photocytotoxicity and low dark cytotoxicity towards Hela, A549, and A375 cells.

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页数：12

相关论文

共 44 条

[1] Photosensitized generation of singlet oxygen from vinyl linked benzo-crown-ether-bipyridyl ruthenium(II) complexes [J].

Abdel-Shafi, AA ;

Beer, PD ;

Mortimer, RJ ;

Wilkinson, F .

JOURNAL OF PHYSICAL CHEMISTRY A, 2000, 104 (02) :192-202

[2] Aryl-Substituted Ruthenium(II) Complexes: A Strategy for Enhanced Photocleavage and Efficient DNA Binding [J].

Abreu, Felipe Diogenes ;

Paulo, Tercio de F. ;

Gehlen, Marcelo H. ;

Ando, Romulo A. ;

Lopes, Luiz G. F. ;

Gondim, Ana Claudia S. ;

Vasconcelos, Mayron A. ;

Teixeira, Edson H. ;

Silva Sousa, Eduardo Henrique ;

Moreira de Carvalho, Idalina Maria .

INORGANIC CHEMISTRY, 2017, 56 (15) :9084-9096

[3] Ruthenium(II) bipyridine complexes with pendant anthracenyl and naphthyl moieties: A strategy for a ROS generator with DNA binding selectivity [J].

Abreu, Felipe Diogenes ;

Nogueira Diogenes, Izaura Cirino ;

de Franca Lopes, Luiz Gonzaga ;

Silva Sousa, Eduardo Henrique ;

Moreira de Carvalho, Idalina Maria .

INORGANICA CHIMICA ACTA, 2016, 439 :92-99

[4] Marked Improvement in Photoinduced Cell Death by a New Tris-heteroleptic Complex with Dual Action: Singlet Oxygen Sensitization and Ligand Dissociation [J].

Albani, Bryan A. ;

Pena, Bruno ;

Leed, Nicholas A. ;

de Paula, Nataly A. B. G. ;

Pavani, Christiane ;

Baptista, Mauricio S. ;

Dunbar, Kim R. ;

Turro, Claudia .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2014, 136 (49) :17095-17101

[5] Ruthenium(II) complexes of redox-related, modified dipyridophenazine ligands: Synthesis, characterization, and DNA interaction [J].

Ambroise, A ;

Maiya, BG .

INORGANIC CHEMISTRY, 2000, 39 (19) :4256-4263

[6] TRIS(PHENANTHROLINE)RUTHENIUM(II) - STEREOSELECTIVITY IN BINDING TO DNA [J].

BARTON, JK ;

DANISHEFSKY, AT ;

GOLDBERG, JM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (07) :2172-2176

[7] Absence of quenching by [Fe(CN)6]4- is not proof of DNA intercalation [J].

Burya, Scott J. ;

Lutterman, Daniel A. ;

Turro, Claudia .

CHEMICAL COMMUNICATIONS, 2011, 47 (06) :1848-1850

[8] A ruthenium(II) arene complex showing emission enhancement and photocleavage activity towards DNA from singlet and triplet excited states respectively [J].

Chen, Yongjie ;

Lei, Wanhua ;

Jiang, Guoyu ;

Zhou, Qianxiong ;

Hou, Yuanjun ;

Li, Chao ;

Zhang, Baowen ;

Wang, Xuesong .

DALTON TRANSACTIONS, 2013, 42 (16) :5924-5931

[9] VISCOSITY AND SEDIMENTATION STUDY OF SONICATED DNA-PROFLAVINE COMPLEXES [J].

COHEN, G ;

EISENBERG, H .

BIOPOLYMERS, 1969, 8 (01) :45-+

[10] Fine-Feature Modifications to Strained Ruthenium Complexes Radically Alter Their Hypoxic Anticancer Activity† [J].

Cole, Houston D. ;

Roque, John A., III ;

Lifshits, Liubov M. ;

Hodges, Rachel ;

Barrett, Patrick C. ;

Havrylyuk, Dmytro ;

Heidary, David ;

Ramasamy, Elamparuthi ;

Cameron, Colin G. ;

Glazer, Edith C. ;

McFarland, Sherri A. .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2022, 98 (01) :73-84

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