A multicenter evaluation of the Abbott Real Time HCV Genotype II assay

被引：32

作者：

Ciotti, Marco ^{[1
]}

Marcuccilli, Fabbio ^{[1
]}

Guenci, Tania ^{[1
]}

Babakir-Mina, Muhammed ^{[1
]}

Chiodo, Federico ^{[2
]}

Favarato, Mose' ^{[3
]}

Perno, Carlo Federico ^{[1
]}

机构：

[1] Fdn Univ Hosp Tor Vergata, Mol Virol Lab, I-00133 Rome, Italy

[2] Ca Granda Niguarda Hosp, Lab Microbiol & Virol, Milan, Italy

[3] UO Mol Biol, Mirano, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 2010年 / 167卷 / 02期

关键词：

HCV genotype; INNO-LiPA; Real-time PCR; HEPATITIS-C-VIRUS; NOMENCLATURE; SUBTYPE;

D O I：

10.1016/j.jviromet.2010.03.017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Genotype determination is recommended before starting anti-HCV therapy to determine the duration of treatment (PEG-Interferon + ribavirin) The Versant HCV Genotype 2.0 assay, based on the reverse hybridization of the 5'UTR segment and core region of hepatitis C virus (HCV), has been one of the assays used most widely for HCV genotyping A multicenter evaluation of the more automated Abbott RealTime HCV Genotype II assay was carried out on 124 HCV positive sera tested previously with the Versant HCV Genotype 2.0 assay. There was good agreement between the two assays Type concordance was 95.9% (117/122) while concordance at the subtype level for genotype 1 was 95.6% (43/45). The Abbott RealTime HCV Genotype II assay is automated, allowing a substantial reduction of time-to results and hands-on time The combined features of full automation, objective interpretation and digital archiving make this assay useful in a diagnostic setting (C) 2010 Elsevier B V. All rights reserved.

引用

页码：205 / 207

页数：3

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