Direct visualization of A-, P-, and E-site transfer RNAs in the Escherichia coli ribosome

被引：187
作者：
Agrawal, RK
Penczek, P
Grassucci, RA
Li, YH
Leith, A
Nierhaus, KH
Frank, J
机构：
[1] NEW YORK STATE DEPT HLTH, WADSWORTH CTR LABS & RES, ALBANY, NY 12201 USA
[2] SUNY ALBANY, DEPT BIOMED SCI, ALBANY, NY 12222 USA
[3] MAX PLANCK INST MOLEC GENET, D-14195 BERLIN, GERMANY
来源：
SCIENCE | 1996年 / 271卷 / 5251期
关键词：
D O I：
10.1126/science.271.5251.1000
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Transfer RNA (tRNA) molecules play a crucial role in protein biosynthesis in all organisms. Their interactions with ribosomes mediate the translation of genetic messages into polypeptides. Three tRNAs bound to the Escherichia coli 70S ribosome were visualized directly with cryoelectron microscopy and three-dimensional reconstruction, The detailed arrangement of A- and P-site tRNAs inferred from this study allows localization of the sites for anticodon interaction and peptide bond formation on the ribosome.
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收藏
页码：1000 / 1002
页数：3
相关论文
共 35 条
[31]  
WATSON JD, 1964, B SOC CHIM BIOL, V46, P1399
[32]   TOPOGRAPHY OF THE E-SITE ON THE ESCHERICHIA-COLI RIBOSOME [J].
WOWER, J ;
SCHEFFER, P ;
SYLVERS, LA ;
WINTERMEYER, W ;
ZIMMERMANN, RA .
EMBO JOURNAL, 1993, 12 (02) :617-623
[33]   RECOMBINANT PHOTOREACTIVE TRANSFER-RNA MOLECULES AS PROBES FOR CROSS-LINKING STUDIES [J].
WOWER, J ;
ROSEN, KV ;
HIXSON, SS ;
ZIMMERMANN, RA .
BIOCHIMIE, 1994, 76 (12) :1235-1246
[34]  
Zhu J. N, UNPUB
[35]  
ZIMMERMANN RA, 1990, RIBOSOME, P331
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