Acceleration of bone formation using in situ-formed hyaluronan-hydrogel containing bone morphogenetic protein-2 in a mouse critical size bone defect model

被引:5
作者
Shoji, Shintaro [1 ]
Uchida, Kentaro [1 ,2 ]
Tazawa, Ryo [1 ]
Saito, Wataru [1 ]
Kuroda, Akiyoshi [1 ]
Sekiguchi, Hiroyuki [1 ,2 ]
Ishii, Daisuke [1 ]
Inoue, Sho [1 ]
Inoue, Gen [1 ]
Takaso, Masashi [1 ]
机构
[1] Kitasato Univ, Dept Orthoped Surg, Sch Med, 1-15-1 Minami Ku Kitasato, Sagamihara, Kanagawa 2520374, Japan
[2] Shonan Univ Med Sci, Res Inst, Nishikubo 500, Chigasaki, Kanagawa 2530083, Japan
关键词
In situ-formed hydrogels; bone defect; carrier; bone morphogenetic protein-2; OPEN TIBIAL FRACTURES;
D O I
10.3233/BME-201172
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
BACKGROUND: An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHGs). IFHGs may also be ideal carrier materials for bone repair, although their ability to carry bone morphogenetic protein-2 (BMP2) has yet to be examined. OBJECTIVE: We examined the effectiveness of an IFHG made of hyaluronan (IFHG-HA) containing BMP2 for promoting bone formation in a mouse critical size bone defect model. METHODS: C57/BL6J mice received a 2-mm femoral critical-sized bone defect before being randomly assigned to one of the following treatment groups (n = 6): control (no treatment), IFHG-HA only, PBS with BMP2, and IFHG-HA with BMP2. X-ray radiographs were utilized to track new bone formation, and micro-computed tomography and histological examination were performed on new bone formed at the bone defect site two weeks after surgery. RESULTS: Mice treated with PBS with BMP2 and IFHG-HA with BMP2 had greater bone volume (BV) and bone mineral content (BMC) than those receiving control, and successfully achieved consolidation. Mice treated with IFHG-HA with BMP2 had significantly higher BV and BMC than those treated with PBS with BMP2. CONCLUSIONS: IFHG-HA may be an effective carrier for BMP2 to enable delivery for bone defect repair.
引用
收藏
页码:207 / 215
页数:9
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