Discrimination of tumor from normal tissues in a mouse model of breast cancer using CARS spectroscopy combined with PC-DFA methodology

Objective methodologies to discriminate tumor from normal tissue in biopsies and resection specimens are of great interest as complementary approaches to existing pathological diagnosis of tumors. In the present study, coherent anti-Stokes Raman scattering (CARS) spectroscopy was applied as an approach to discriminate resected tumor from normal mammary tissue in murine mammary tumor virus-Wnt-1 transgenic mouse model of breast cancer. Due to the dense CH molecular vibration in the range from 2500 to 3100cm(-1), the classification was performed by using principal component-discriminant function analysis to discriminate tumor from the normal tissue. A total of 240 training and 40 testing CARS spectra were acquired. The overall accuracy of CARS, based on cross-validation and external validation method, was 98% and 95%, respectively. The present study demonstrates a diagnostic method with a 1-s spectral acquirement rate, using a CARS spectroscopic technique. Our results suggest that CARS combined with the principal component-discriminant function analysis is a potentially useful tool for identification and classification of breast cancer tissues. Copyright (c) 2017 John Wiley & Sons, Ltd.