Development of novel magnetic nano-carriers for high-performance affinity purification

被引：75

作者：

Nishio, Kosuke ^{[1
]}

Masaike, Yuka ^{[2
]}

Ikeda, Morihito ^{[1
]}

Narimatsu, Hiroki ^{[1
]}

Gokon, Nobuyuki ^{[1
]}

Tsubouchi, Shingo ^{[1
]}

Hatakeyama, Mamoru ^{[2
]}

Sakamoto, Satoshi ^{[1
]}

Hanyu, Naohiro ^{[3
]}

Sandhu, Adarsh ^{[4
]}

Kawaguchi, Haruma ^{[5
]}

Abe, Masanori ^{[6
]}

Handa, Hiroshi ^{[1
,2
]}

机构：

[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat, Midori Ku, Yokohama, Kanagawa 2268501, Japan

[2] Tokyo Inst Technol, Integrated Res Inst, Yokohama, Kanagawa 2268501, Japan

[3] Tamagawa Seiki Co Ltd, Motortron Lab, Nagano 3958520, Japan

[4] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Elect & Elect Engn, Meguro Ku, Tokyo 1528550, Japan

[5] Keio Univ, Dept Appl Chem, Yokohama, Kanagawa 2238522, Japan

[6] Tokyo Inst Technol, Grad Sch Sci & Engn, Dept Phys Elect, Meguro Ku, Tokyo 1528552, Japan

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2008年 / 64卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

magnetite; polymerization; magnetic carrier; affinity purification; drug target;

D O I：

10.1016/j.colsurfb.2008.01.013

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We developed novel magnetic nano-carriers around 180 mn in diameter for affinity purification. Prepared magnetic nano-carriers possessed uniform core/shell/shell nano-structure composed of 40 nm magnetite particles/poly(styrene-co-glycidyl methacrylate (GMA))/polyGMA, which was constructed by admicellar polymerization. By utilizing relatively large 40 nm magnetite particles with large magnetization, the magnetic nanocarriers could show good response to permanent magnet. Thanks to uniform polymer shell with high physical/chemical stability, the magnetic nano-carriers could disperse in a wide range of organic solvent without disruption of core/shell structure and could immobilize various kinds of drugs. We examined affinity purification using our prepared magnetic nano-carriers with anti-cancer agent methotrexate (MTX) as ligand. Our magnetic nano-carriers showed higher performance compared to commercially available magnetic beads in terms of purification efficiency of target including extent of non-specific binding protein. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：162 / 169

页数：8

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