An interactive framework for whole-brain maps at cellular resolution

被引：146

作者：

Furth, Daniel ^{[1
]}

Vaissiere, Thomas ^{[2
]}

Tzortzi, Ourania ^{[1
]}

Xuan, Yang ^{[1
]}

Martin, Antje ^{[1
]}

Lazaridis, Iakovos ^{[1
]}

Spigolon, Giada ^{[1
]}

Fisone, Gilberto ^{[1
]}

Tomer, Raju ^{[3
]}

Deisseroth, Karl ^{[3
]}

Carlen, Marie ^{[1
]}

Miller, Courtney A. ^{[2
,4
]}

Rumbaugh, Gavin ^{[2
]}

Meletis, Konstantinos ^{[1
]}

机构：

[1] Karolinska Inst, Dept Neurosci, Stockholm, Sweden

[2] Scripps Res Inst, Dept Neurosci, Jupiter, FL USA

[3] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[4] Scripps Res Inst, Dept Mol Med, Jupiter, FL USA

来源：

NATURE NEUROSCIENCE | 2018年 / 21卷 / 01期

基金：

瑞典研究理事会;

关键词：

GENE-EXPRESSION; SCALE; RECONSTRUCTION; DECOMPOSITION; POPULATIONS; CONNECTOME; ACTIVATION; STRATEGIES; SECTIONS; SINGLE;

D O I：

10.1038/s41593-017-0027-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To deconstruct the architecture and function of brain circuits, it is necessary to generate maps of neuronal connectivity and activity on a whole-brain scale. New methods now enable large-scale mapping of the mouse brain at cellular and subcellular resolution. We developed a framework to automatically annotate, analyze, visualize and easily share whole-brain data at cellular resolution, based on a scale-invariant, interactive mouse brain atlas. This framework enables connectivity and mapping projects in individual laboratories and across imaging platforms, as well as multiplexed quantitative information on the molecular identity of single neurons. As a proof of concept, we generated a comparative connectivity map of five major neuron types in the corticostriatal circuit, as well as an activity-based map to identify hubs mediating the behavioral effects of cocaine. Thus, this computational framework provides the necessary tools to generate brain maps that integrate data from connectivity, neuron identity and function.

引用

页码：139 / +

页数：15

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