B7-H1 and B7-DC receptors of oral squamous carcinoma cells are upregulated by Porphyromonas gingivalis

被引：106

作者：

Groeger, Sabine ^{[1
]}

Domann, Eugen ^{[2
]}

Gonzales, Jose R. ^{[1
]}

Chakraborty, Trinad ^{[2
]}

Meyle, Joerg ^{[1
]}

机构：

[1] Univ Giessen, Dept Periodontol, Zentrum Zahn Mund & Kieferheilkunde, D-35392 Giessen, Germany

[2] Univ Giessen, Inst Med Mikrobiol, D-35392 Giessen, Germany

来源：

IMMUNOBIOLOGY | 2011年 / 216卷 / 12期

关键词：

B7-H1; Death receptor; Porphyromonas gingivalis; Squamous carcinoma cells; Upregulation; STREPTOCOCCUS-SALIVARIUS K12; GASTRIC EPITHELIAL-CELLS; T-CELL; B7; FAMILY; POTENTIAL MECHANISM; MEMBRANE-VESICLES; IFN-GAMMA; IN-VITRO; EXPRESSION; CANCER;

D O I：

10.1016/j.imbio.2011.05.005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The up-regulation of the B7-H1 receptors in host cells might influence the chronicity of inflammatory disorders that frequently precede the development of human cancers. B7-H1 expression has been detected in the majority of human cancers, leading to anergy and apoptosis of activated T cells, and enabling tumor cells to overcome host response. Porphyromonas gingivalis (P. gingivalis), a putative periodontal pathogen, is an etiologic agent of periodontitis and expresses a variety of virulence factors. In this study, the expression of B7-H1 and B7-DC receptors on squamous cell carcinoma cells SCC-25 and BHY and primary human gingival keratinocytes (PHGK) was analyzed after infection with two virulent P. gingivalis strains in vitro. After 48 h, the cells were stained with antibodies for human B7-H1 and B7-DC and further analyzed by flow cytometry. RNA was extracted and gene expression of B7-H1 or B7-DC was quantified by real time PCR. After infection with P. gingivalis, both B7-H1 and B7-DC receptors were up-regulated. The mean fluorescence intensity (MFI) increased from 4.5 to 9.9 (B7-H1) and from 6.9 to 15.0 (B7-DC) (p < 0.05, respectively) in SCC-25 cells. PHGK showed an increase from 4.8 to 12.4 (B7-H1) and from 5.5 to 15.6 (B7-DC) (p < 0.05, respectively). Streptococcus salivarius K12, a commensal bacterium, caused no up-regulation. After 24 h, the expression of B7H1 and B7-DC mRNA in infected cells, normalized to GAPDH and in relation to non-infected cells, was 6.4 fold (B7-H1) and 8.6 fold (B7-DC) higher. In PHGK B7-H1/DC mRNA expression increased 8.2 fold (B7-H1) and 5.9 fold (B7DC) (p < 0.05) respectively. The results of the study demonstrate that in contrast to S. salivarius K12 virulent P. gingivalis strains are able to induce the expression of the B7-H1 and B7-DC receptors in squamous carcinoma cells and human gingival keratinocytes, which might facilitate immune evasion by oral cancers. (C) 2011 Elsevier GmbH. All rights reserved.

引用

页码：1302 / 1310

页数：9

共 82 条

[41] PD-L2 is a second ligand for PD-I and inhibits T cell activation [J].

Latchman, Y ;

Wood, CR ;

Chernova, T ;

Chaudhary, D ;

Borde, M ;

Chernova, I ;

Iwai, Y ;

Long, AJ ;

Brown, JA ;

Nunes, R ;

Greenfield, EA ;

Bourque, K ;

Boussiotis, VA ;

Carter, LL ;

Carreno, BM ;

Malenkovich, N ;

Nishimura, H ;

Okazaki, T ;

Honjo, T ;

Sharpe, AH ;

Freeman, GJ .

NATURE IMMUNOLOGY, 2001, 2 (03) :261-268

[42] Regulation of PD-1, PD-L1, and PD-L2 expression during normal and autoimmune responses [J].

Liang, SC ;

Latchman, YE ;

Buhlmann, JE ;

Tomczak, MF ;

Horwitz, BH ;

Freeman, GJ ;

Sharpe, AH .

EUROPEAN JOURNAL OF IMMUNOLOGY, 2003, 33 (10) :2706-2716

[43] Plasma cells from multiple myeloma patients express B7-H1 (PD-L1) and increase expression after stimulation with IFN-γ and TLR ligands via a MyD88-, TRAF6-, and MEK-dependent pathway [J].

Liu, Jizhong ;

Hamrouni, Abdelbasset ;

Wolowiec, Darius ;

Coiteux, Valrie ;

Kuliczkowski, Kazirnierz ;

Hetuin, Dominique ;

Saudemont, Aurore ;

Quesnel, Bruno .

BLOOD, 2007, 110 (01) :296-304

[44] BIOLOGICAL-ACTIVITIES OF OUTER-MEMBRANE VESICLES [J].

MAYRAND, D ;

GRENIER, D .

CANADIAN JOURNAL OF MICROBIOLOGY, 1989, 35 (06) :607-613

[45] Risk of oral cancer associated with tobacco smoking, alcohol consumption and oral hygiene:: a case-control study in Madrid, Spain [J].

Moreno-López, LA ;

Esparza-Gómez, GC ;

González-Navarro, A ;

Cerero-Lapiedra, R ;

González-Hernández, MJ ;

Domínguez-Rojas, V .

ORAL ONCOLOGY, 2000, 36 (02) :170-174

[46] Prokaryotic regulation of epithelial responses by inhibition of IκB-α ubiquitination [J].

Neish, AS ;

Gewirtz, AT ;

Zeng, H ;

Young, AN ;

Hobert, ME ;

Karmali, V ;

Rao, AS ;

Madara, JL .

SCIENCE, 2000, 289 (5484) :1560-1563

[47] A role for fimbriae in Porphyromonas gingivalis invasion of oral epithelial cells [J].

Njoroge, T ;

Genco, RJ ;

Sojar, HT ;

Hamada, N ;

Genco, CA .

INFECTION AND IMMUNITY, 1997, 65 (05) :1980-1984

[48] Clinical significance of programmed death-1 ligand-1 and programmed death-1 ligand-2 expression in human esophageal cancer [J].

Ohigashi, Y ;

Sho, M ;

Yamada, Y ;

Tsurui, Y ;

Hamada, K ;

Ikeda, N ;

Mizuno, T ;

Yoriki, R ;

Kashizuka, H ;

Yane, K ;

Tsushima, F ;

Otsuki, N ;

Yagita, H ;

Azuma, M ;

Nakajima, Y .

CLINICAL CANCER RESEARCH, 2005, 11 (08) :2947-2953

[49] Blockade of B7-H1 or B7-DC induces an anti-tumor effect in a mouse pancreatic cancer model [J].

Okudaira, Keisuke ;

Hokari, Ryota ;

Tsuzuki, Yoshikazu ;

Okada, Yoshikiyo ;

Komoto, Shunsuke ;

Watanabe, Chikako ;

Kurihara, Chie ;

Kawaguchi, Atsushi ;

Nagao, Shigeaki ;

Azuma, Miyuki ;

Yagita, Hideo ;

Miura, Soichiro .

INTERNATIONAL JOURNAL OF ONCOLOGY, 2009, 35 (04) :741-749

[50] TLR4 signaling induces B7-H1 expression through MAPK pathways in bladder cancer cells [J].

Qian, Yigang ;

Deng, Junfang ;

Geng, Lei ;

Xie, Haiyang ;

Jiang, Guoping ;

Zhou, Lin ;

Wang, Yan ;

Yin, Shenyong ;

Feng, Xiaowen ;

Liu, Junwei ;

Ye, Zhou ;

Zheng, Shusen .

CANCER INVESTIGATION, 2008, 26 (08) :816-821

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