Isolation and molecular characterisation of Mycobacterium bovis from raw milk in Tunisia

被引:0
作者
Ben Kahla, I [1 ,2 ]
Boschiroli, M. L. [3 ]
Souissi, F. [2 ]
Cherif, N. [2 ]
Benzarti, M. [4 ]
Boukadida, J. [1 ]
Hammami, S. [2 ]
机构
[1] CHU Farhat Hached, Microbiol & Immunol Lab, Sousse 4001, Tunisia
[2] Vet Res Inst Tunisia, Tunis 1006, Tunisia
[3] French Agcy Food Environm & Occupat Hlth Safety, Natl & OIE FAO Bovine TB Reference Labs, Bacterial Zoonoses Unit, Anim Hlth Lab, F-94706 Maisons Alfort, France
[4] Natl Vet Med Sch, Sidi Thabet, Tunisia
关键词
Mycobacterium bovis; Tuberculosis; Raw milk; Zoonosis; TUBERCULOSIS COMPLEX; CATTLE; IDENTIFICATION; STRAINS;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Consumption of raw milk and unpasteurized dairy products is common in Tunisia where bovine tuberculosis remains enzootic. We herein investigated the frequency of M. bovis isolation from raw milk. Methods: Three hundred and six milk samples collected from 102 infected cows in different Tunisian regions were analysed. M. bovis isolates were further characterized by spoligotyping and variable number tandem repeat typing. Results: A total of five (4.9 %) M. bovis strains exhibiting three different genotypes were isolated. Conclusion: This study demonstrates that consumers of raw milk or derivatives in Tunisia are at high risk of zoonotic infection with M. bovis.
引用
收藏
页码:S2 / S5
页数:4
相关论文
共 50 条
[21]   Comparative study of Mycobacterium bovis primary isolation methods [J].
Issa, Marina de Azeuedo ;
Soares Filho, Paulo Martins ;
Fonseca Junior, Antonio Augusto ;
Hodon, Mikael Arrais ;
dos Santos, Lilian Cristian ;
Pimenta dos Reis, Jenner Karlisson ;
Leite, Romulo Cerqueira .
BRAZILIAN JOURNAL OF MICROBIOLOGY, 2017, 48 (01) :139-144
[22]   Mycobacterium bovis, but also M. africanum present in raw milk of pastoral cattle in north-central Nigeria [J].
Cadmus, Simeon I. B. ;
Yakubu, Mohammed K. ;
Magaji, Abdullahi A. ;
Jenkins, Akinbowale O. ;
van Soolingen, Dick .
TROPICAL ANIMAL HEALTH AND PRODUCTION, 2010, 42 (06) :1047-1048
[23]   Molecular characterisation of Mycobacterium bovis isolates from cattle carcases at a city slaughterhouse in Uganda [J].
Asiimwe, B. B. ;
Asiimwe, J. ;
Kallenius, G. ;
Ashaba, F. K. ;
Ghebremichael, S. ;
Joloba, M. ;
Koivula, T. .
VETERINARY RECORD, 2009, 164 (21) :655-658
[24]   Multiple strains of Mycobacterium bovis isolates identified by molecular typing of bovine animals slaughtered in slaughterhouse [J].
Alzamora Filho, Fernando ;
Vasconcellos, Sidra E. G. ;
Gomes, Harrison M. ;
Cavalcante, Miriam P. ;
Suffys, Philip N. ;
Costa, Joselito N. .
PESQUISA VETERINARIA BRASILEIRA, 2014, 34 (02) :103-108
[25]   Survival kinetics of Mycobacterium bovis during manufacture and ripening of raw milk Cheddar and Caerphilly cheese produced on a laboratory-scale [J].
Forgrave, R. ;
Donaghy, J. A. ;
Fisher, A. ;
Rowe, M. T. .
JOURNAL OF APPLIED MICROBIOLOGY, 2016, 121 (05) :1457-1468
[26]   Mycobacterium bovis detection from milk of negative skin test cows [J].
Zarden, C. F. O. ;
Marassi, C. D. ;
Figueiredo, E. E. E. S. ;
Lilenbaum, W. .
VETERINARY RECORD, 2013, 172 (05) :130-+
[27]   Effectiveness of Mycobacterium paratuberculosis isolation from raw milk by means of direct isolation of DNA and classic culture [J].
Szteyn, J. ;
Wismiewska-Laszczych, A. ;
Rusmynska, A. .
POLISH JOURNAL OF VETERINARY SCIENCES, 2008, 11 (01) :25-28
[28]   Molecular genotyping and epidemiology of Mycobacterium bovis strains obtained from cattle in Iran [J].
Mosavari, Nader ;
Feizabadi, Mohammad Mehdi ;
Jamshidian, Mahmoud ;
Shahpouri, Masoud Reza Seifiabadi ;
Forbes, Ken J. ;
Pajoohi, Reza Aref ;
Keshavarz, Rouholah ;
Taheri, Mohammad Mohammad ;
Tadayon, Keyvan .
VETERINARY MICROBIOLOGY, 2011, 151 (1-2) :148-152
[29]   Molecular analysis of clinical isolates of Mycobacterium bovis recovered from humans in Italy [J].
Lari, Nicoletta ;
Rindi, Laura ;
Bonanni, Daniela ;
Tortoli, Enrico ;
Garzelli, Carlo .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (11) :4218-4221
[30]   Interlaboratory test: Isolation of Mycobacterium bovis from granulomatous lesions in bovine [J].
Garbaccio, Sergio ;
Barandiaran, Soledad ;
Fernandez, Analia ;
Macias, Analia ;
Magnano, Gabriel ;
Martinez Vivot, Marcela ;
Peyru, Maite ;
Cataldi, Angel .
REVISTA ARGENTINA DE MICROBIOLOGIA, 2016, 48 (02) :161-165