Isolation and molecular characterisation of Mycobacterium bovis from raw milk in Tunisia

被引:0
作者
Ben Kahla, I [1 ,2 ]
Boschiroli, M. L. [3 ]
Souissi, F. [2 ]
Cherif, N. [2 ]
Benzarti, M. [4 ]
Boukadida, J. [1 ]
Hammami, S. [2 ]
机构
[1] CHU Farhat Hached, Microbiol & Immunol Lab, Sousse 4001, Tunisia
[2] Vet Res Inst Tunisia, Tunis 1006, Tunisia
[3] French Agcy Food Environm & Occupat Hlth Safety, Natl & OIE FAO Bovine TB Reference Labs, Bacterial Zoonoses Unit, Anim Hlth Lab, F-94706 Maisons Alfort, France
[4] Natl Vet Med Sch, Sidi Thabet, Tunisia
关键词
Mycobacterium bovis; Tuberculosis; Raw milk; Zoonosis; TUBERCULOSIS COMPLEX; CATTLE; IDENTIFICATION; STRAINS;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Consumption of raw milk and unpasteurized dairy products is common in Tunisia where bovine tuberculosis remains enzootic. We herein investigated the frequency of M. bovis isolation from raw milk. Methods: Three hundred and six milk samples collected from 102 infected cows in different Tunisian regions were analysed. M. bovis isolates were further characterized by spoligotyping and variable number tandem repeat typing. Results: A total of five (4.9 %) M. bovis strains exhibiting three different genotypes were isolated. Conclusion: This study demonstrates that consumers of raw milk or derivatives in Tunisia are at high risk of zoonotic infection with M. bovis.
引用
收藏
页码:S2 / S5
页数:4
相关论文
共 11 条
[1]   Spacer oligonucleotide typing of Mycobacterium bovis strains from cattle and other animals: A tool for studying epidemiology of tuberculosis [J].
Aranaz, A ;
Liebana, E ;
Mateos, A ;
Dominguez, L ;
Vidal, D ;
Domingo, M ;
Gonzolez, O ;
RodriguezFerri, EF ;
Bunschoten, AE ;
VanEmbden, JDA ;
Cousins, D .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (11) :2734-2740
[2]  
Bahri S, 1991, BAYTARI, V3, P1
[3]   Zoonotic tuberculosis due to Mycobacterium bovis in developing countries [J].
Cosivi, O ;
Grange, JM ;
Daborn, CJ ;
Raviglione, MC ;
Fujikura, T ;
Cousins, D ;
Robinson, RA ;
Huchzermeyer, HFAK ;
de Kantor, I ;
Meslin, FX .
EMERGING INFECTIOUS DISEASES, 1998, 4 (01) :59-70
[4]   PCR-based method to differentiate the subspecies of the Mycobacterium tuberculosis complex on the basis of genomic deletions [J].
Huard, RC ;
Lazzarini, LCD ;
Butler, WR ;
van Soolingen, D ;
Ho, JL .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (04) :1637-1650
[5]   Isolation and identification of mycobacteria from livestock specimens and milk obtained in Brazil [J].
Leite, CQF ;
Anno, IS ;
Leite, SR ;
Roxo, E ;
Morlock, GP ;
Cooksey, RC .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2003, 98 (03) :319-323
[6]   Direct detection and identification of Mycobacterium tuberculosis and mycobacterium bovis in bovine samples by a novel nested PCR assay:: Correlation with conventional techniques [J].
Mishra, A ;
Singhal, A ;
Chauhan, DS ;
Katoch, VM ;
Srivastava, K ;
Thakral, SS ;
Bharadwaj, SS ;
Sreenivas, V ;
Prasad, HK .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (11) :5670-5678
[7]   Development of variable-number tandem repeat typing of Mycobacterium bovis:: Comparison of results with those obtained by using existing exact tandem repeats and spoligotyping [J].
Roring, S ;
Scott, A ;
Brittain, D ;
Walker, I ;
Hewinson, G ;
Neill, S ;
Skuce, R .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (06) :2126-2133
[8]   Molecular characterization of Mycobacterium bovis strains isolated from cattle slaughtered at two abattoirs in Algeria [J].
Sahraoui, Naima ;
Mueller, Borna ;
Guetarni, Djamel ;
Boulahbal, Fadela ;
Yala, Djamel ;
Ouzrout, Rachid ;
Berg, Stefan ;
Smith, Noel H. ;
Zinsstag, Jakob .
BMC VETERINARY RESEARCH, 2009, 5
[9]  
Srivastava K, 2008, INDIAN J MED RES, V128, P26
[10]   The importance of Mycobacterium bovis as a zoonosis [J].
Thoen, C ;
LoBue, P ;
de Kantor, I .
VETERINARY MICROBIOLOGY, 2006, 112 (2-4) :339-345