Polysaccharides Extracted From Panax Ginseng CA Mey Enhance Complement Component 4 Biosynthesis in Human Hepatocytes

被引:13
作者
Liu, Shuang [1 ]
Liu, Fangbing [1 ]
Wang, Tingting [1 ]
Liu, Jianzeng [2 ]
Hu, Cheng [1 ]
Sun, Liwei [3 ]
Wang, Guan [1 ]
机构
[1] Jilin Univ, Sch Life Sci, Natl Engn Lab AIDS Vaccine, Key Lab Mol Enzymol & Engn, Changchun, Peoples R China
[2] Changchun Univ Chinese Med, Jilin Ginseng Acad, Changchun, Peoples R China
[3] Changchun Univ Chinese Med, Affiliated Hosp, Res Ctr Tradit Chinese Med, Key Lab Act Subst & Biol Mech Ginseng Efficacy, Changchun, Peoples R China
关键词
ginseng; water-soluble ginseng polysaccharides; complement component 4; C4; transcription; promoter; IMMUNOSTIMULATORY ACTIVITY; TRANSCRIPTION; C4; RECOGNITION; IMMUNITY; LEAVES;
D O I
10.3389/fphar.2021.734394
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Panax ginseng C.A. Mey (ginseng) is a classic medicinal plant which is well known for enhancing immune capacity. Polysaccharides are one of the main active components of ginseng. We isolated water-soluble ginseng polysaccharides (WGP) and analyzed the physicochemical properties of WGP including molecular weight, monosaccharide composition, and structural characteristics. WGP had minimal effect on the growth of hepatocytes. Interestingly, WGP significantly increased the mRNA and protein levels of complement component 4 (C4), one of the core components of the complement system. Promoter reporter gene assays revealed that WGP significantly enhanced activity of the C4 gene promoter. Deletion analyses determined that the E-box1 and Sp1 regions play key roles in WGP-induced C4 transcription. Taken together, our results suggest that WGP promotes C4 biosynthesis through upregulation of transcription. These results provide new explanation for the intrinsic mechanism by which ginseng boosts human immune capacity.
引用
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页数:9
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共 43 条
[1]   The different faces of Notch in T-helper-cell differentiation [J].
Amsen, Derk ;
Antov, Andrey ;
Flavell, Richard A. .
NATURE REVIEWS IMMUNOLOGY, 2009, 9 (02) :116-124
[2]   Transcriptional Repression of C4 Complement by Hepatitis C Virus Proteins [J].
Banerjee, Arup ;
Mazumdar, Budhaditya ;
Meyer, Keith ;
Di Bisceglie, Adrian M. ;
Ray, Ratna B. ;
Ray, Ranjit .
JOURNAL OF VIROLOGY, 2011, 85 (09) :4157-4166
[3]   A MOLECULAR MAP OF THE HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-III REGION LINKING COMPLEMENT GENES-C4, GENE-C2 AND FACTOR-B [J].
CARROLL, MC ;
CAMPBELL, RD ;
BENTLEY, DR ;
PORTER, RR .
NATURE, 1984, 307 (5948) :237-241
[4]   STRUCTURE AND ORGANIZATION OF THE C4-GENES [J].
CARROLL, MC ;
BELT, T ;
PALSDOTTIR, A ;
PORTER, RR .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1984, 306 (1129) :379-388
[5]   Regulation of Humoral Immunity by Complement [J].
Carroll, Michael C. ;
Isenman, David E. .
IMMUNITY, 2012, 37 (02) :199-207
[6]  
Carter R.H., 2010, SCIENCE, V184, P2233
[7]   Laser microdissection hyphenated with high performance gel permeation chromatography-charged aerosol detector and ultra performance liquid chromatography-triple quadrupole mass spectrometry for histochemical analysis of polysaccharides in herbal medicine: Ginseng, a case study [J].
Chen, Qi-Lei ;
Chen, Yu-Jie ;
Zhou, Shan-Shan ;
Yip, Ma-Man ;
Xu, Jun ;
Chen, Hu-Biao ;
Zhao, Zhong-Zhen .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 107 :332-342
[8]   Complement, infection, and autoimmunity [J].
Conigliaro, Paola ;
Triggianese, Paola ;
Ballanti, Eleonora ;
Perricone, Carlo ;
Perricone, Roberto ;
Chimenti, Maria Sole .
CURRENT OPINION IN RHEUMATOLOGY, 2019, 31 (05) :532-541
[9]   Androgen Signaling in Prostate Cancer [J].
Dai, Charles ;
Heemers, Hannelore ;
Sharifi, Nima .
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE, 2017, 7 (09)
[10]   THE COMPLEMENT COMPONENT C4 OF MAMMALS [J].
DODDS, AW ;
LAW, SKA .
BIOCHEMICAL JOURNAL, 1990, 265 (02) :495-502