Measurement of protein size in concentrated solutions by small angle X-ray scattering

被引：5
|
作者：
Liu, Jun [1 ]
Li, Zhihong [1 ]
Wei, Yanru [1 ]
Wang, Wenjia [1 ]
Wang, Bing [2 ]
Liang, Hongli [2 ]
Gao, Yuxi [2 ]
机构：
[1] Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China
[2] Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China
来源：
PROTEIN SCIENCE | 2016年 / 25卷 / 08期
关键词：
protein size; inter-particle interference; distance distribution function; small angle X-ray scattering; LIGHT-SCATTERING; SAXS;
D O I：
10.1002/pro.2957
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
By simulations on the distance distribution function (DDF) derived from small angle X-ray scattering (SAXS) theoretical data of a dense monodisperse system, we found a quantitative mathematical correlation between the apparent size of a spherically symmetric (or nearly spherically symmetric) homogenous particle and the concentration of the solution. SAXS experiments on protein solutions of human hemoglobin and horse myoglobin validated the correlation. This gives a new method to determine, from the SAXS DDF, the size of spherically symmetric (or nearly spherically symmetric) particles of a dense monodisperse system, specifically for protein solutions with interference effects.
引用
收藏
页码：1385 / 1389
页数：5
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