An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum

被引：45

作者：

Bock, Thomas ^{[1
]}

Chen, Wei-Hua ^{[1
]}

Ori, Alessandro ^{[1
]}

Malik, Nayab ^{[1
]}

Silva-Martin, Noella ^{[1
]}

Huerta-Cepas, Jaime ^{[1
]}

Powell, Sean T. ^{[1
]}

Kastritis, Panagiotis L. ^{[1
]}

Smyshlyaev, Georgy ^{[1
,2
]}

Vonkova, Ivana ^{[1
]}

Kirkpatrick, Joanna ^{[3
]}

Doerks, Tobias ^{[1
]}

Nesme, Leo ^{[1
]}

Bassler, Jochen ^{[4
]}

Kos, Martin ^{[4
]}

Hurt, Ed ^{[4
]}

Carlomagno, Teresa ^{[1
]}

Gavin, Anne-Claude ^{[1
]}

Barabas, Orsolya ^{[1
]}

Mueller, Christoph W. ^{[1
]}

van Noort, Vera ^{[1
]}

Beck, Martin ^{[1
]}

Bork, Peer ^{[1
]}

机构：

[1] EMBL, Struct & Computat Biol Unit, D-69117 Heidelberg, Germany

[2] Inst Cytol & Genet, Lab Mol Genet Syst, Novosibirsk 630090, Russia

[3] EMBL, Prote Core Facil, D-69117 Heidelberg, Germany

[4] Heidelberg Univ, Biochem Zentrum, D-69120 Heidelberg, Germany

来源：

NUCLEIC ACIDS RESEARCH | 2014年 / 42卷 / 22期

关键词：

MASS-SPECTROMETRY; STRUCTURAL BASIS; PROTEOME; COMPLEX; QUANTIFICATION; EXPRESSION; ORGANISMS;

D O I：

10.1093/nar/gku1147

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The thermophilic fungus Chaetomium thermophilum holds great promise for structural biology. To increase the efficiency of its biochemical and structural characterization and to explore its thermophilic properties beyond those of individual proteins, we obtained transcriptomics and proteomics data, and integrated them with computational annotation methods and a multitude of biochemical experiments conducted by the structural biology community. We considerably improved the genome annotation of Chaetomium thermophilum and characterized the transcripts and expression of thousands of genes. We furthermore show that the composition and structure of the expressed proteome of Chaetomium thermophilum is similar to its mesophilic relatives. Data were deposited in a publicly available repository and provide a rich source to the structural biology community.

引用

页码：13525 / 13533

页数：9

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