Crystal structure of a family I.3 lipase from Pseudomonas sp MIS38 in a closed conformation

被引：81

作者：

Angkawidjaja, Clement

You, Dong-Ju

Matsumura, Hiroyoshi

Kuwahara, Katsumasa

Koga, Yuichi

Takano, Kazufumi

Kanaya, Shigenori

机构：

[1] Osaka Univ, Grad Sch Engn, Dept Mat & Life Sci, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Engn, Dept Appl Chem, Osaka 5650871, Japan

[3] JST, CRESTO, Osaka 5650871, Japan

来源：

FEBS LETTERS | 2007年 / 581卷 / 26期

关键词：

family i.3 lipase; crystal structure; closed; conformation; lid; Ca2+; binding site; pseudomonas sp; MIS38;

D O I：

10.1016/j.febslet.2007.09.048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5 angstrom resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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页码：5060 / 5064

页数：5

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