High-speed 3D imaging based on structured illumination and electrically tunable lens

被引:7
作者
Wang, Dongping [1 ,2 ]
Meng, Yunlong [1 ,2 ]
Chen, Dihan [1 ,2 ]
Yam, Yeung [1 ]
Chen, Shih-Chi [1 ,2 ]
机构
[1] Chinese Univ Hong Kong, Dept Mech & Automat Engn, Shatin, Hong Kong, Peoples R China
[2] Chinese Univ Hong Kong, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
基金
中国国家自然科学基金;
关键词
MICROSCOPY; LIVE; CELLS;
D O I
10.3788/COL201715.090004
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
In this Letter, we present a high-speed volumetric imaging system based on structured illumination and an electrically tunable lens (ETL), where the ETL performs fast axial scanning at hundreds of Hz. In the system, a digital micro-mirror device (DMD) is utilized to rapidly generate structured images at the focal plane in synchronization with the axial scanning unit. The scanning characteristics of the ETL are investigated theoretically and experimentally. Imaging experiments on pollen samples are performed to verify the optical cross-sectioning and fast axial scanning capabilities. The results show that our system can perform fast axial scanning and three-dimensional (3D) imaging when paired with a high-speed camera, presenting an economic solution for advanced biological imaging applications.
引用
收藏
页数:4
相关论文
共 50 条
  • [41] Motion-Based Structure Separation for Label-Free High-Speed 3-D Cardiac Microscopy
    Bhat, Sandeep
    Ohn, Jungho
    Liebling, Michael
    IEEE TRANSACTIONS ON IMAGE PROCESSING, 2012, 21 (08) : 3638 - 3647
  • [42] Dual GRIN lens two-photon endoscopy for high-speed volumetric and deep brain imaging
    Chien, Yu-Feng
    Lin, Jyun-Yi
    Yeh, Po-Ting
    Hsu, Kuo-Jen
    Tsai, Yu-Hsuan
    Chen, Shih-Kuo
    Chu, Shi-Wei
    BIOMEDICAL OPTICS EXPRESS, 2021, 12 (01) : 162 - 172
  • [43] Structured illumination for 3-D Mie imaging and 2-D attenuation measurements in optically dense sprays
    Kristensson, Elias
    Berrocal, Edouard
    Wellander, Rikard
    Ritcher, Mattias
    Alden, Marcus
    Linne, Mark
    PROCEEDINGS OF THE COMBUSTION INSTITUTE, 2011, 33 : 855 - 861
  • [44] High Speed 3D Endoscopic Optical Frequency Domain Imaging Probe for Lung Cancer Diagnosis
    Li, Jianan
    Feroldi, Fabio
    Mo, Jianhua
    Helderman, Frank
    de Groot, Mattijs
    de Boer, Johannes F.
    OPTICAL COHERENCE TOMOGRAPHY AND COHERENCE TECHNIQUES VI, 2013, 8802
  • [45] Video-rate volumetric neuronal imaging using 3D targeted illumination
    Xiao, Sheng
    Tseng, Hua-an
    Gritton, Howard
    Han, Xue
    Mertz, Jerome
    SCIENTIFIC REPORTS, 2018, 8
  • [46] 3D Super-Resolution Reconstruction Using Microsphere-Assisted Structured Illumination Microscopy
    Xie, Zhongye
    Hu, Song
    Tang, Yan
    Liu, Xi
    Liu, Junbo
    He, Yu
    IEEE PHOTONICS TECHNOLOGY LETTERS, 2019, 31 (22) : 1783 - 1786
  • [47] High-speed scanning chromatic confocal sensor for 3-D imaging with modeling-free learning control
    Ito, Shingo
    Poik, Mathias
    Csencsics, Ernst
    Schlarp, Johannes
    Schitter, Georg
    APPLIED OPTICS, 2020, 59 (29) : 9234 - 9242
  • [48] 3D Imaging Based on Depth Measurement Technologies
    Chen, Ni
    Zuo, Chao
    Lam, Edmund Y.
    Lee, Byoungho
    SENSORS, 2018, 18 (11)
  • [49] High refractive index immersion liquid for superresolution 3D imaging using sapphire-based aplanatic numerical aperture increasing lens optics
    Laskar, Junaid M.
    Kumar, P. Shravan
    Herminghaus, Stephan
    Daniels, Karen E.
    Schroeter, Matthias
    APPLIED OPTICS, 2016, 55 (12) : 3165 - 3169
  • [50] Digital Micromirror Device-based High-speed and High-resolution Quantitative Phase Imaging
    Zheng, Cheng
    Zhou, Renjie
    EMERGING DIGITAL MICROMIRROR DEVICE BASED SYSTEMS AND APPLICATIONS XI, 2019, 10932