An offline two-dimensional supercritical fluid chromatography x reversed phase liquid chromatography tandem quadrupole time-of-flight mass spectrometry system for comprehensive gangliosides profiling in swine brain extract

被引:20
作者
Si, Wei [1 ,2 ]
Liu, Yanfang [1 ]
Xiao, Yuansheng [1 ]
Guo, Zhimou [1 ]
Jin, Gaowa [1 ]
Yan, Jingyu [1 ]
Shen, Aijin [1 ]
Zhou, Han [1 ]
Yang, Fan [1 ]
Liang, Xinmiao [1 ]
机构
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100000, Peoples R China
基金
美国国家科学基金会;
关键词
Offline two-dimensional liquid chromatography; Supercritical fluid chromatography x reversed phase liquid chromatography; In-house library; Self-developed software; Quadrupole time-of-flight mass spectrometry; Swine brain extract; CARTHAMUS-TINCTORIUS L; HIGH-THROUGHPUT; PATHOGENIC ROLE; IONIZATION; CHIP; IDENTIFICATION; METABOLISM; SULFATIDES; MEDICINE; MARKERS;
D O I
10.1016/j.talanta.2019.120366
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Gangliosides, widely distributed in tissues and body fluid, have been connected to the therapy of cancer and brain related diseases. The complexity of the gangliosides structures with different polar moieties coexisting, a carbohydrate moiety and a ceramide chain, make it a great challenge in separation and analysis science. This study aimed to develop a strategy on the basis of high-accuracy data collected by an offline two-dimensional (2D) supercritical fluid chromatography (SFC) x reversed phase liquid chromatography (RPLC)/quadrupole time-of-flight (Q-ToF) system, and to integrate an in-house library with self-developed software for fast screening and identification of gangliosides from a complex sample (swine brain extract). Subsequent positive-mode MS/MS was used to validate the identified gangliosides. Finally, 153 gangliosides were separated and 79 of them were identified by the in-house library and self-developed software, 4-fold more than those by manual identification (18 gangliosides). Among the identified ones, 20 were detected in swine brain for the first time. This study established an offline 2D SFC x RPLC system and provided a new method for fast screening and automatic identification of gangliosides in complex mixtures. It will be conducive to further study of biological functions of gangliosides.
引用
收藏
页数:9
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