Cloning, expression and characterization of the ornithine decarboxylase gene from Dictyostelium discoideum

被引：4

作者：

Kumar, Rishikesh ^{[1
]}

Rafia, Sheikh ^{[1
]}

Saran, Shweta ^{[1
]}

机构：

[1] Jawaharlal Nehru Univ, Sch Life Sci, New Delhi 110067, India

来源：

INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY | 2014年 / 58卷 / 09期

关键词：

discoideum; ODC expression; putrescine; INDUCED AUTOPHAGY; POLYAMINES; SPERMIDINE; INDUCTION; DIFFERENTIATION; PUTRESCINE; CELLS;

D O I：

10.1387/ijdb.140174ss

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ornithine decarboxylase (ODC) is a rate limiting enzyme in polyamine synthesis that decarboxylates ornithine to form the diamine putrescine. We report here the isolation, expression and characterization of a homolog of ODC from Dictyostelium discoideum. DdODC is conserved and shows sequence and structural homology with that from human. Both ODC transcript and protein are expressed at all stages of development and show high expression in prestalk/stalk cells. It is cytosolic and predominantly perinuclear in localization. Both overexpression of DdODC and putrescine treatment resulted in inhibition of cell proliferation.

引用

页码：669 / 676

页数：8

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