Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

被引：109

作者：

Chiriaco, Maria Serena ^{[1
]}

Bianco, Monica ^{[1
]}

Nigro, Annamaria ^{[2
]}

Primiceri, Elisabetta ^{[1
]}

Ferrara, Francesco ^{[1
,3
]}

Romano, Alessandro ^{[2
]}

Quattrini, Angelo ^{[2
]}

Furlan, Roberto ^{[2
]}

Arima, Valentina ^{[1
]}

Maruccio, Giuseppe ^{[1
,4
]}

机构：

[1] CNR NANOTEC Inst Nanotechnol, Via Monteroni, I-73100 Lecce, Italy

[2] Ist Sci San Raffaele, Inst Expt Neurol, Div Neurosci, I-20132 Milan, Italy

[3] STMicroelectronics, Via Monteroni, I-73100 Lecce, Italy

[4] Univ Salento, Dept Math & Phys, Via Monteroni, I-73100 Lecce, Italy

来源：

SENSORS | 2018年 / 18卷 / 10期

基金：

欧盟地平线“2020”;

关键词：

lab-on-chip; microfabrication; microfluidics; biosensors; extracellular vesicles; exosomes; CELL-DERIVED MICROVESICLES; CONTINUOUS PARTICLE SEPARATION; HYBRIDIZATION CHAIN-REACTION; EXTRACELLULAR VESICLES; PANCREATIC-CANCER; GENOMIC DNA; ELECTROCHEMICAL DETECTION; MYELOID MICROVESICLES; SIZE CHARACTERIZATION; MICROFLUIDIC DEVICE;

D O I：

10.3390/s18103175

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge potential as biomarkers in a high number of disorders and pathologies as they are considered as carriers of information among cells, as well as being responsible for the spreading of diseases. Current methods of analysis of microvesicles and exosomes do not fulfill the requirements for their in-depth investigation and the complete exploitation of their diagnostic and prognostic value. Lab-on-chip methods have the potential and capabilities to bridge this gap and the technology is mature enough to provide all the necessary steps for a completely automated analysis of extracellular vesicles in body fluids. In this paper we provide an overview of the biological role of extracellular vesicles, standard biochemical methods of analysis and their limits, and a survey of lab-on-chip methods that are able to meet the needs of a deeper exploitation of these biological entities to drive their use in common clinical practice.

引用

页数：41

共 245 条

[11] Radiochemistry on chip: towards dose-on-demand synthesis of PET radiopharmaceuticals [J].

Arima, Valentina ;

Pascali, Giancarlo ;

Lade, Oliver ;

Kretschmer, Hans R. ;

Bernsdorf, Ingo ;

Hammond, Victoria ;

Watts, Paul ;

De Leonardis, Francesco ;

Tarn, Mark D. ;

Pamme, Nicole ;

Cvetkovic, Benjamin Z. ;

Dittrich, Petra S. ;

Vasovic, Nikola ;

Duane, Russell ;

Jaksic, Aleksandar ;

Zacheo, Antonella ;

Zizzari, Alessandra ;

Marra, Lucia ;

Perrone, Elisabetta ;

Salvadori, Piero A. ;

Rinaldi, Rosaria .

LAB ON A CHIP, 2013, 13 (12) :2328-2336

[12] Fluoropolymers coatings on polydimethylsiloxane for retarding swelling in toluene [J].

Arima, Valentina ;

Bianco, Monica ;

Zacheo, Antonella ;

Zizzari, Alessandra ;

Perrone, Elisabetta ;

Marra, Lucia ;

Rinaldi, Ross .

THIN SOLID FILMS, 2012, 520 (06) :2293-2300

[13] A simple flow cytometry method improves the detection of phosphatidylserine-exposing extracellular vesicles [J].

Arraud, N. ;

Gounou, C. ;

Linares, R. ;

Brisson, A. R. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2015, 13 (02) :237-247

[14] Depletion of microglia and inhibition of exosome synthesis halt tau propagation [J].

Asai, Hirohide ;

Ikezu, Seiko ;

Tsunoda, Satoshi ;

Medalla, Maria ;

Luebke, Jennifer ;

Haydar, Tank ;

Wolozin, Benjamin ;

Butovsky, Oleg ;

Kuegler, Sebastian ;

Ikezu, Tsuneya .

NATURE NEUROSCIENCE, 2015, 18 (11) :1584-1593

[15] Determination of the size distribution of blood microparticles directly in plasma using atomic force microscopy and microfluidics [J].

Ashcroft, B. A. ;

de Sonneville, J. ;

Yuana, Y. ;

Osanto, S. ;

Bertina, R. ;

Kuil, M. E. ;

Oosterkamp, T. H. .

BIOMEDICAL MICRODEVICES, 2012, 14 (04) :641-649

[16] Mesenchymal stem cell secreted vesicles provide novel opportunities in (stem) cell-free therapy [J].

Baglio, Serena Rubina ;

Pegtel, D. Michiel ;

Baldini, Nicola .

FRONTIERS IN PHYSIOLOGY, 2012, 3

[17] Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods [J].

Baranyai, Tamas ;

Herczeg, Kata ;

Onodi, Zsofia ;

Voszka, Istvan ;

Modos, Karoly ;

Marton, Nikolett ;

Nagy, Gyoergy ;

Maeger, Imre ;

Wood, Matthew J. ;

El Andaloussi, Samir ;

Palinkas, Zoltan ;

Kumar, Vikas ;

Nagy, Pater ;

Kittel, Agnes ;

Buzas, Edit Iren ;

Ferdinandy, Peter ;

Giricz, Zoltan .

PLOS ONE, 2015, 10 (12)

[18] Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer [J].

Barat, David ;

Spencer, Daniel ;

Benazzi, Giuseppe ;

Mowlem, Matthew Charles ;

Morgan, Hywel .

LAB ON A CHIP, 2012, 12 (01) :118-126

[19] Proteomic analysis of exosomes isolated from human malignant pleural effusions [J].

Bard, MP ;

Hegmans, JP ;

Hemmes, A ;

Luider, TM ;

Willemsen, R ;

Severijnen, LAA ;

van Meerbeeck, JP ;

Burgers, SA ;

Hoogsteden, HC ;

Lambrecht, BN .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2004, 31 (01) :114-121

[20] Acid sphingomyelinase activity triggers microparticle release from glial cells [J].

Bianco, Fabio ;

Perrotta, Cristiana ;

Novellino, Luisa ;

Francolini, Maura ;

Riganti, Loredana ;

Menna, Elisabetta ;

Saglietti, Laura ;

Schuchman, Edward H. ;

Furlan, Roberto ;

Clementi, Emilio ;

Matteoli, Michela ;

Verderio, Claudia .

EMBO JOURNAL, 2009, 28 (08) :1043-1054

← 1 2 3 4 5 6 7 8 9 10 →