Assessment of two flexible and compatible SNP genotyping platforms:: TaqMan® SNP genotyping assays and the SNPlex™ genotyping system

被引：191

作者：

De La Vega, FA ^{[1
]}

Lazaruk, KD ^{[1
]}

Rhodes, MD ^{[1
]}

Wenz, MH ^{[1
]}

机构：

[1] Appl Biosyst Inc, Foster City, CA 94404 USA

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2005年 / 573卷 / 1-2期

关键词：

single nucleotide polymorphism; SNP; genotyping; association studies; linkage mapping; linkage disequilibrium; 5 '-nuclease; TaqMan; oligonucleotide ligation; OLA; ZipCode; pharmacogenetics;

D O I：

10.1016/j.mrfmmm.2005.01.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this review we describe the principles, protocols, and applications of two commercially available SNP genotyping platforms, the TaqMan (R) SNP Genotyping Assays and the SNPlex (TM) Genotyping System. Combined, these two technologies meet the requirements of multiple SNP applications in genetics research and pharmacogenetics. We also describe a set of SNP selection tools and validated assay resources which we developed to accelerate the cycle of experimentation on these platforms. Criteria for selecting the more appropriate of these two genotyping technologies are presented: the genetic architecture of the trait of interest, the throughput required, and the number of SNPs and samples needed for a successful study. Overall, the TaqMan assay format is suitable for low- to mid-throughput applications in which a high assay conversion rate, simple assay workflow, and low cost of automation are desirable. The SNPlex Genotyping System, on the other hand, is well suited for SNP applications in which throughput and cost-efficiency are essential, e.g., applications requiring either the testing of large numbers of SNPs and samples. or the flexibility to select various SNP subsets. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：111 / 135

页数：25

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