ANTIMICROBIAL ACTIVITY OF ENDOPHYTIC FUNGUS FUSARIUM SP ISOLATED FROM MEDICINAL HONEYSUCKLE PLANT

被引：14

作者：

Zhang, Huiru ^{[1
]}

Sun, Xinchen ^{[2
]}

Xu, Chunping ^{[2
]}

机构：

[1] Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Henan, Peoples R China

[2] Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Henan, Peoples R China

来源：

ARCHIVES OF BIOLOGICAL SCIENCES | 2016年 / 68卷 / 01期

关键词：

Endophyte; antimicrobial activity; Fusarium sp; culture broth; FLOS-LONICERAE; ESCHERICHIA-COLI;

D O I：

10.2298/ABS140401004Z

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Endophytes of plants have a wide range of strains comprising important sources of various bioactive metabolites. An endophytic fungus was isolated from honeysuckle, an important Chinese medicinal plant. The phylogenetic and physiological characterization indicated that the isolated strain JY2 corresponded to Fusarium sp. The culture filtrate of JY2 displayed antagonism activity against some pathogenic bacteria owing to the existence of antimicrobial compounds. The filtrate revealed the strongest in vitro antimicrobial activity on Pseudomonas aeruginosa by increasing the permeability of cell membranes. The antibacterial extract was fractionated and purified using silica gel chromatography. Five different bioactive compounds were isolated by bioactivity-guided fractionation from the culture extracts of JY2, and preliminarily identified by HPLC-MS spectral data. These results suggest that Fusarium sp. features a potentially remarkable antimicrobial activity and could be valuable to discover the new drugs or agents for antimicrobial purposes.

引用

页码：25 / 30

页数：6

共 16 条

[1] Fungal endophytes: unique plant inhabitants with great promises [J].

Aly, Amal Hassan ;

Debbab, Abdessamad ;

Proksch, Peter .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2011, 90 (06) :1829-1845

[2]

[Anonymous], 1980, FEDDES REPERT

[3] Quality evaluation of Flos Lonicerae through a simultaneous determination of seven saponins by HPLC with ELSD [J].

Chai, XY ;

Li, SL ;

Li, P .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1070 (1-2) :43-48

[4] Qualitative and quantitative analysis of active flavonoids in Flos Lonicerae by capillary zone electrophoresis coupled with solid-phase extraction [J].

Chen, J ;

Li, SL ;

Li, P ;

Song, Y ;

Chai, XY ;

Ma, DY .

JOURNAL OF SEPARATION SCIENCE, 2005, 28 (04) :365-372

[5]

Gong LJ, 2009, AFR J BIOTECHNOL, V8, P731

[6] Isolation and characterization of antagonistic bacteria against bacterial leaf spot of Euphorbia pulcherrima [J].

Li, B. ;

Xu, L. H. ;

Lou, M. M. ;

Li, F. ;

Zhang, Y. D. ;

Xie, G. L. .

LETTERS IN APPLIED MICROBIOLOGY, 2008, 46 (04) :450-455

[7] Determination of five maior iridoid glucosides in Flos Lonicerae by high-performance liquid chromatography coupled with evaporative light scattering detection [J].

Li, HJ ;

Li, P ;

Ye, WC .

JOURNAL OF CHROMATOGRAPHY A, 2003, 1008 (02) :167-172

[8] RELEASE OF ALKALINE PHOSPHATASE FROM CELLS OF ESCHERICHIA COLI UPON LYSOZYME SPHEROPLAST FORMATION [J].

MALAMY, MH ;

HORECKER, BL .

BIOCHEMISTRY, 1964, 3 (12) :1889-&

[9] BIOLOGICALLY-ACTIVE SECONDARY METABOLITES OF ENDOPHYTIC PEZICULA SPECIES [J].

SCHULZ, B ;

SUCKER, J ;

AUST, HJ ;

KROHN, K ;

LUDEWIG, K ;

JONES, PG ;

DORING, D .

MYCOLOGICAL RESEARCH, 1995, 99 :1007-1015

[10]

Seeber F., 2008, J MICROBIOL METH, V39, P189

← 1 2 →