Directed breeding of an Arthrobacter mutant for high-yield production of cyclic adenosine monophosphate by N+ ion implantation

被引：16

作者：

Song, He ^{[1
]}

Chen, Xiaochun ^{[1
]}

Cao, Jiaming ^{[1
]}

Fang, Ting ^{[1
]}

Bai, Jianxin ^{[1
]}

Xiong, Jian ^{[1
]}

Ying, Hanjie ^{[1
]}

机构：

[1] Nanjing Univ Technol, State Key Lab Mat Oriented Chem Engn, Coll Life Sci & Pharmaceut Engn, Nanjing 210009, Peoples R China

来源：

RADIATION PHYSICS AND CHEMISTRY | 2010年 / 79卷 / 08期

关键词：

Arthrobacter A302; Cyclic adenosine monophosphate; N+ ion implantation; Mutation breeding; Enzymatic analysis; BACILLUS-SUBTILIS; ADENYLATE-CYCLASE; ACID; STRAIN; IRRADIATION; BEAM;

D O I：

10.1016/j.radphyschem.2010.03.005

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

To obtain a cyclic adenosine monophosphate (cAMP) high-yield production strain, Arthrobacter NG-1 was mutated by N+ ion implantation with an energy level of 10 key and dose of 7 x 10(15) ions/cm(2). Combined with directed screening methods, a xanthine-defective and 8-azaguanine (8-AG)-resistant mutant Arthrobacter A302 was selected. The concentration of cAMP produced by this mutant was 41.7% higher than that of the original strain and reached 9.78 g/L. Through ten-generation investigation, the capability of cAMP production of A302 was found to be stable. Compared with the original strain, the special activities of key enzymes in A302, which influenced the CAMP biosynthesis, was analyzed. IMP dehydrogenase activity was defective, whereas PRPP amidotransferase, sAMP synthetase and adenylate cyclase activities were increased by 61.5%, 147% and 21.7%, respecitively, which might explain the mutagenesis mechanism by N+ ions implantation under the enzymatic level. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：826 / 830

页数：5

共 23 条

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