Interaction of the protein phosphatase 2A with the regulatory domain of the cystic fibrosis transmembrane conductance regulator channel

被引：17

作者：

Vastiau, A

Cao, LS

Jaspers, M

Owsianik, G

Janssens, V

Cuppens, H

Goris, J

Nilius, B

Cassiman, JJ

机构：

[1] Katholieke Univ Leuven, Dept Human Genet, Div Human Mutat & Polymorphisms, B-3000 Louvain, Belgium

[2] Katholieke Univ Leuven, Dept Mol Cell Biol, Div Physiol, B-3000 Louvain, Belgium

[3] Katholieke Univ Leuven, Dept Mol Cell Biol, Div Biochem, B-3000 Louvain, Belgium

来源：

FEBS LETTERS | 2005年 / 579卷 / 16期

关键词：

CFTR; PP2A; protein-protein interaction;

D O I：

10.1016/j.febslet.2005.04.079

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A direct interaction of the regulatory domain (R domain) of the cystic fibrosis transmembrane conductance regulator protein (CFTR) with PR65, a regulatory subunit of the protein phosphatase 2A (PP2A), was shown in yeast two hybrid, pull-down and co-immunoprecipitation experiments. The R domain could be dephosphorylated by PP2A in vitro. Overexpression of the interacting domain of PR65 in Caco-2 cells, as well as treatment with okadaic acid, showed a prolonged deactivation of the chloride channel. Taken together our results show a direct and functional interaction between CFTR and PP2A. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3392 / 3396

页数：5

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