Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens

被引：4

作者：

Ignatova, Nadezhda ^{[1
]}

Ivanova, Tatiana ^{[2
]}

Antonyan, Artem ^{[3
]}

Budruev, Ivan ^{[4
]}

Streltsova, Olga ^{[3
]}

Elagin, Vadim ^{[5
]}

Kamensky, Vladislav ^{[5
,6
]}

机构：

[1] Privolzhsky Res Med Univ, Dept Epidemiol Microbiol & Evidence Based Med, 10-1 Minin & Pozharsky Sq, Nizhnii Novgorod 603005, Russia

[2] Privolzhsky Res Med Univ, Med & Preventat Care Dept, 10-1 Minin & Pozharsky Sq, Nizhnii Novgorod 603005, Russia

[3] Privolzhsky Res Med Univ, Dept Urol Named Shakhov, 10-1 Minin & Pozharsky Sq, Nizhnii Novgorod 603005, Russia

[4] Natl Res Lobachevsky State Univ Nizhny Novgorod, Inst Biol & Biomed, 23 Prospekt Gagarina Gagarin Ave, Nizhnii Novgorod 603022, Russia

[5] Privolzhsky Res Med Univ, Inst Expt Oncol & Biomed Technol, 10-1 Minin & Pozharsky Sq, Nizhnii Novgorod 603005, Russia

[6] Russian Acad Sci, Fed Res Ctr Inst Appl Phys, 46 Ulyanov St, Nizhnii Novgorod 603950, Russia

来源：

PHOTONICS | 2021年 / 8卷 / 11期

基金：

俄罗斯科学基金会;

关键词：

urolithiasis; antibiotic-resistant bacteria; bactericidal effect; laser-induced inactivation; photosensitizer; EPIDEMIOLOGY; INFECTION; PERMEABILITY; MANAGEMENT; DIFFUSION; STONES;

D O I：

10.3390/photonics8110495

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Photodynamic inactivation (PDI) is considered to be an effective method of prevention of postoperative complications of urolithiasis. The present study shows a complex approach to assess the efficacy of PDI of drug resistant bacteria associated with renal calculi. Bacterial strains associated with renal calculi were isolated and identified using standard methods of bacteriological analysis and tested for drug resistance to 10 antibiotics by the disco-diffusion method. Uropathogenic bacterial strains present in 78.7 & PLUSMN; 5.2% of the infected samples from the total number of analyzed calculi. The most frequent representatives belonged to the genera Staphylococcus, Escherichia, and Enterococcus. All tested strains showed high antibiotic resistance. Representatives of the most common bacterial genera in the calculi were used as models for the selection of PD exposure modes. It was found that the maximum time of photosensitizer accumulation depends on the structure of the bacterial cell wall: 30 min for Gram-negative strains and 60 min for Gram-positive ones. Optimal modes of PD exposure to antibiotic-resistant uropathogenic microorganisms were selected: 50 mu g/mL Fotoditazin and 150 mW laser power. The maximal bactericidal activity of PDI against uropathogenic microorganisms was shown for Enterococcus faecalis, and Staphylococcus aureus. The bacteriostatic effect was found against Escherichia coli and Proteus mirabilis.

引用

页数：11

共 42 条

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