N-Cadherin Maintains the Healthy Biology of Nucleus Pulposus Cells under High-Magnitude Compression

被引:8
作者
Wang, Zhenyu [1 ]
Leng, Jiali [2 ]
Zhao, Yuguang [3 ]
Yu, Dehai [3 ]
Xu, Feng [1 ]
Song, Qingxu [1 ]
Qu, Zhigang [1 ]
Zhuang, Xinming [1 ]
Liu, Yi [1 ]
机构
[1] Jilin Univ, Hosp 1, Dept Spinal Surg, Changchun, Jilin, Peoples R China
[2] Jilin Univ, Hosp 1, Nursing Platform Bone Joint & Sports Med, Changchun, Jilin, Peoples R China
[3] Jilin Univ, Hosp 1, Canc Ctr, Changchun, Jilin, Peoples R China
基金
中国国家自然科学基金;
关键词
Disc degeneration; N-cadherin; Nucleus pulposus; Matrix; Compression; Apoptosis; INTERVERTEBRAL DISC DEGENERATION; MECHANICALLY ACTIVE BIOREACTOR; DYNAMIC COMPRESSION; MATRIX SYNTHESIS; PHENOTYPE; DIFFERENTIATION; EXPRESSION; STRATEGIES; MARKERS; SYSTEM;
D O I
10.1159/000484385
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Background/Aims: Mechanical load can regulate disc nucleus pulposus (NP) biology in terms of cell viability, matrix homeostasis and cell phenotype. N-cadherin (N-CDH) is a molecular marker of NP cells. This study investigated the role of N-CDH in maintaining NP cell phenotype, NP matrix synthesis and NP cell viability under high-magnitude compression. Methods: Rat NP cells seeded on scaffolds were perfusion-cultured using a self-developed perfusion bioreactor for 5 days. NP cell biology in terms of cell apoptosis, matrix biosynthesis and cell phenotype was studied after the cells were subjected to different compressive magnitudes (low-and high-magnitudes: 2% and 20% compressive deformation, respectively). Non-loaded NP cells were used as controls. Lentivirus-mediated N-CDH overexpression was used to further investigate the role of N-CDH under high-magnitude compression. Results: The 20% deformation compression condition significantly decreased N-CDH expression compared with the 2% deformation compression and control conditions. Meanwhile, 20% deformation compression increased the number of apoptotic NP cells, up-regulated the expression of Bax and cleaved-caspase-3 and down-regulated the expression of Bcl-2, matrix macromolecules (aggrecan and collagen II) and NP cell markers (glypican-3, CAXII and keratin-19) compared with 2% deformation compression. Additionally, N-CDH overexpression attenuated the effects of 20% deformation compression on NP cell biology in relation to the designated parameters. Conclusion: N-CDH helps to restore the cell viability, matrix biosynthesis and cellular phenotype of NP cells under high-magnitude compression. (C) 2017 The Author(s) Published by S. Karger AG, Basel
引用
收藏
页码:2327 / 2337
页数:11
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