Strand-specific postreplicative processing of mammalian telomeres

被引：218
作者：
Bailey, SM
Cornforth, MN
Kurimasa, A
Chen, DJ
Goodwin, EH
机构：
[1] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87544 USA
[2] Univ Texas, Med Branch, Dept Radiat Oncol, Galveston, TX 77550 USA
[3] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Cell & Mol Biol, Berkeley, CA 94720 USA
来源：
SCIENCE | 2001年 / 293卷 / 5539期
关键词：
D O I：
10.1126/science.1062560
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Telomeres are specialized nucleoprotein structures that stabilize the ends of linear eukaryotic chromosomes. In mammalian cells, abrogation of telomeric repeat binding factor TRF2 or DNA-dependent protein kinase (DNA-PK) activity causes end-to-end chromosomal fusion, thus establishing an essential role for these proteins in telomere function. Here we show that TRF2-mediated endcapping occurs after telomere replication. The postreplicative requirement for TRF2 and DNA-PKcs, the catalytic subunit of DNA-PK, is confined to only half of the telomeres, namely, those that were produced by leading-strand DNA synthesis. These results demonstrate a crucial difference in postrepticative processing of telomeres that is linked to their mode of replication.
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页码：2462 / 2465
页数：4
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