Early Thyroid Hormone-induced Gene Expression Changes in N2a-β Neuroblastoma Cells

被引：4

作者：

Bedo, Gabriela ^{[1
]}

Pascual, Angel ^{[2
]}

Aranda, Ana ^{[2
]}

机构：

[1] Univ Republica, Secc Genet Evolut, Fac Ciencias, Montevideo 11400, Uruguay

[2] CSIC, Inst Invest Biomed, E-28029 Madrid, Spain

来源：

JOURNAL OF MOLECULAR NEUROSCIENCE | 2011年 / 45卷 / 02期

关键词：

Thyroid hormones; Gene expression profile; Neuroblastoma cells; Array; ELEMENT-BINDING PROTEIN; REAL-TIME PCR; STATISTICAL-ANALYSIS; RESPONSIVE GENES; NERVOUS-SYSTEM; NEURO-2A CELLS; ONTO-TOOLS; RECEPTOR; DIFFERENTIATION; TRANSCRIPTION;

D O I：

10.1007/s12031-010-9389-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thyroid hormone has long been known to regulate neural development. Hypothyroidism during pregnancy and early postnatal period has severe neurological consequences including even mental retardation. The purpose of this study was to characterize gene expression pattern during thyroid hormone-induced differentiation of neuro-2a beta cells in order to select "direct response genes" for further analysis. In this neuroblastoma cell line, thyroid hormone blocks proliferation and induces differentiation. Changes in gene expression level were examined after a T3 treatment of 3 and 24 h using cDNA arrays. Sixteen genes were significantly up-regulated and 79 down-regulated by T3 treatment. Five up-regulated genes not previously described as regulated by thyroid hormone and selected for their putative significance to understand T3 action on cell differentiation, were verified by RT-PCR analysis. The transcription factors Phox2a and basic helix-loop-helix domain containing, class B2 mRNAs exhibited a clear increase after 3- and 24-h treatment. The guanine-nucleotide exchange factor RalGDS was greatly up-regulated after 3-h treatment but not 24 h after. The results suggest an early involvement of these genes in T3 action during neuroblastoma cell differentiation probably mediating later changes in gene expression pattern.

引用

页码：76 / 86

页数：11

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