Periplasmic penicillin G acylase activity in recombinant Escherichia coli cells permeabilized with organic solvents

被引：41
作者：
De León, A
García, B
de la Rosa, APB
Villaseñor, F
Estrada, A
López-Revilla, R
机构：
[1] Inst Potosino Invest Cientif & Tecnol, Dept Mol Biol, San Luis Potosi 78231, SLP, Mexico
[2] Inst Tecnol Celaya, Celaya 38040, Gto, Mexico
[3] Univ Autonoma San Luis Potosi, San Luis Potosi 78210, SLP, Mexico
来源：
PROCESS BIOCHEMISTRY | 2003年 / 39卷 / 03期
关键词：
organic solvent; penneabilization; penicillin G acylase; dielectric constant; Hydrophobicity; recombinant; E; coli;
D O I：
10.1016/S0032-9592(03)00079-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recombinant Escherichia coli cells expressing penicillin G acylase (PGA) were permeabilized with organic solvents. Using the aromatic solvents, hexane or chloroform, increases of specific enzyme activity up to 380% were obtained, whereas acetone and pyridine reduced drastically activity and alcohols inactivated the enzyme. The type of solvent, physicochemical properties, solvent concentration and time of permeabilization were evaluated to obtain the optimal conditions to increase PGA activity by cell permeabilization. Solvents with lower dielectric constants (<5) and high hydrophobicity (log P > 2) were more effective in increasing PGA activity. (C) 2003 Elsevier Science Ltd. All rights reserved.
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收藏
页码：301 / 305
页数：5
相关论文
共 25 条
  • [1] SOLVENT DIELECTRIC EFFECTS ON PROTEIN DYNAMICS
    AFFLECK, R
    HAYNES, CA
    CLARK, DS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) : 5167 - 5170
  • [2] Interaction of penicillin V acylase with organic solvents:: catalytic activity modulation on the hydrolysis of penicillin V
    Arroyo, M
    Torres, R
    de la Mata, I
    Castillón, MP
    Acebal, C
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 1999, 25 (3-5) : 378 - 383
  • [3] *ASI INSTR INC, 2001, DIEL CONST REF GUID
  • [4] ISOLATION AND KINETICS OF PENICILLIN AMIDASE FROM ESCHERICHIA-COLI
    BALASINGHAM, K
    WARBURTON, D
    DUNNILL, P
    LILLY, MD
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 276 (01) : 250 - +
  • [5] STUDIES OF OPERATIONAL VARIABLES IN BATCH MODE FOR GENETICALLY-ENGINEERED ESCHERICHIA-COLI-CELLS CONTAINING PENICILLIN ACYLASE
    BHATTACHARYA, S
    GUPTA, VS
    PRABHUNE, AA
    SIVARAMAN, H
    DEBNATH, M
    RANJEKAR, PK
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 1993, 15 (12) : 1070 - 1073
  • [6] A postfermentative stage improves penicillin acylase production by a recombinant E-coli
    DeLeon, A
    Galindo, E
    Ramirez, OT
    [J]. BIOTECHNOLOGY LETTERS, 1996, 18 (08) : 927 - 932
  • [7] FERROLUZZI AG, 1984, J BACTERIOL, V16, P1181
  • [8] PERMEABILIZATION OF YEAST-CELLS (KLUYVEROMYCES) WITH ORGANIC-SOLVENTS
    FLORES, MV
    VOGET, CE
    ERTOLA, RJJ
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 1994, 16 (04) : 340 - 346
  • [9] Permeabilization of Pichia pastoris for glycolate oxidase activity
    Gough, S
    Deshpande, M
    Scher, M
    Rosazza, JPN
    [J]. BIOTECHNOLOGY LETTERS, 2001, 23 (18) : 1535 - 1537
  • [10] ACTIVITY AND FLEXIBILITY OF ALCOHOL-DEHYDROGENASE IN ORGANIC-SOLVENTS
    GUINN, RM
    SKERKER, PS
    KAVANAUGH, P
    CLARK, DS
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 1991, 37 (04) : 303 - 308
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