The DNA methyltransferase DNMT3C protects male germ cells from transposon activity

被引：275

作者：

Barau, Joan ^{[1
]}

Teissandier, Aurelie ^{[1
,2
]}

Zamudio, Natasha ^{[1
,7
]}

Roy, Stephanie ^{[3
]}

Nalesso, Valerie ^{[4
,5
,6
]}

Herault, Yann ^{[4
,5
,6
]}

Guillou, Florian ^{[3
]}

Bourc'his, Deborah ^{[1
]}

机构：

[1] PSL Res Univ, Inst Curie, INSERM, CNRS, F-75005 Paris, France

[2] Ecole Mines, F-75005 Paris, France

[3] Univ Tours, IFCE, CNRS, PRC,INRA, F-37380 Nouzilly, France

[4] Strasbourg Univ, Inst Genet & Biol Mol & Cellulaire, F-67404 Illkirch Graffenstaden, France

[5] CNRS, UMR7104, Illkirch Graffenstaden, France

[6] INSERM, U964, Illkirch Graffenstaden, France

[7] Walter & Eliza Hall Inst Med Res, Parkville, Vic, Australia

来源：

SCIENCE | 2016年 / 354卷 / 6314期

基金：

欧洲研究理事会;

关键词：

DE-NOVO METHYLATION; MOUSE; GENOME; RETROTRANSPOSONS; PARASITES; RODENTS; GUIDES; FAMILY; MICE; RNA;

D O I：

10.1126/science.aah5143

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA methylation is prevalent in mammalian genomes and plays a central role in the epigenetic control of development. The mammalian DNA methylation machinery is thought to be composed of three DNA methyltransferase enzymes (DNMT1, DNMT3A, and DNMT3B) and one cofactor (DNMT3L). Here, we describe the discovery of Dnmt3C, a de novo DNA methyltransferase gene that evolved via a duplication of Dnmt3B in rodent genomes and was previously annotated as a pseudogene. We show that DNMT3C is the enzyme responsible for methylating the promoters of evolutionarily young retrotransposons in the male germ line and that this specialized activity is required for mouse fertility. DNMT3C reveals the plasticity of the mammalian DNA methylation system and expands the scope of the mechanisms involved in the epigenetic control of retrotransposons.

引用

页码：909 / 912

页数：4

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