Changes in GABAergic Transmission to and Intrinsic Excitability of Gonadotropin-Releasing Hormone (GnRH) Neurons during the Estrous Cycle in Mice

被引:24
作者
Adams, Caroline [1 ]
Chen, Xi [1 ]
Moenter, Suzanne M. [1 ,2 ,3 ]
机构
[1] Univ Michigan, Dept Mol & Integrat Physiol, 7725 Med Sci 2,1137 East Catherine St, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Internal Med, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Obstet & Gynecol, Ann Arbor, MI 48109 USA
关键词
action potential; estradiol; excitability; feedback; GABA; GnRH; LUTEINIZING-HORMONE; ESTRADIOL FEEDBACK; POTASSIUM CURRENTS; CALCIUM-CHANNELS; SURGE; SECRETION; STIMULATION; EXPRESSION; INDUCTION; OVULATION;
D O I
10.1523/ENEURO.0171-18.2018
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Gonadotropin-releasing hormone (GnRH) neurons form the final common central output pathway controlling fertility and are regulated by steroid feedback. In females, estradiol feedback action varies between negative and positive; negative feedback typically regulates episodic GnRH release whereas positive feedback initiates a surge of GnRH, and subsequently luteinizing hormone (LH) release ultimately triggering ovulation. During the estrous cycle, changes between estradiol negative and positive feedback occur with cycle stage and time of day, with positive feedback in the late afternoon of proestrus in nocturnal species. To test the hypotheses that synaptic and intrinsic properties of GnRH neurons are regulated by cycle stage and time of day, we performed whole-cell patch-clamp studies of GnRH neurons in brain slices from mice at two times considered negative feedback (diestrous PM and proestrous AM) and during positive feedback (proestrous PM). GABAergic transmission can excite GnRH neurons and was higher in cells from proestrous PM mice than cells from proestrous AM mice and approached traditional significance levels relative to cells from diestrous PM mice. Action potential response to current injection was also greater in cells from proestrous PM mice than the other two groups. Interestingly, the hormonal milieu of proestrous AM provided stronger negative feedback on both GnRH neuron excitability and GABAergic postsynaptic current (PSC) amplitude than diestrous PM. These observations demonstrate elements of both synaptic and intrinsic properties of GnRH neurons are regulated in a cycle-dependent manner and provide insight into the neurobiological mechanisms underlying cyclic changes in neuroendocrine function among states of estradiol negative and positive feedback.
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页数:10
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共 44 条
[1]   Gonadotropin-Releasing Hormone (GnRH) Neuron Excitability Is Regulated by Estradiol Feedback and Kisspeptin [J].
Adams, Caroline ;
Stroberg, Wylie ;
DeFazio, Richard A. ;
Schnell, Santiago ;
Moenter, Suzanne M. .
JOURNAL OF NEUROSCIENCE, 2018, 38 (05) :1249-1263
[2]   Quantal currents at single-site central synapses [J].
Auger, C ;
Marty, A .
JOURNAL OF PHYSIOLOGY-LONDON, 2000, 526 (01) :3-11
[3]   SEASONAL-CHANGES OF GONADOTROPIN-RELEASING-HORMONE SECRETION IN THE EWE [J].
BARRELL, GK ;
MOENTER, SM ;
CARATY, A ;
KARSCH, FJ .
BIOLOGY OF REPRODUCTION, 1992, 46 (06) :1130-1135
[5]   CONTROL OF THE PREOVULATORY RELEASE OF LUTEINIZING-HORMONE BY STEROIDS IN THE MOUSE [J].
BRONSON, FH ;
VOMSAAL, FS .
ENDOCRINOLOGY, 1979, 104 (05) :1247-1255
[7]   Dendritic Spine Plasticity in Gonadatropin-Releasing Hormone (GnRH) Neurons Activated at the Time of the Preovulatory Surge [J].
Chan, Heidi ;
Prescott, Melanie ;
Ong, ZhiYi ;
Herde, Michel K. ;
Herbison, Allan E. ;
Campbell, Rebecca E. .
ENDOCRINOLOGY, 2011, 152 (12) :4906-4914
[8]   Absence of gonadotropin surges and gonadotropin-releasing hormone self-priming in ovariectomized (OVX), estrogen (E2)-treated, progesterone receptor knockout (PRKO) mice [J].
Chappell, PE ;
Schneider, JS ;
Kim, P ;
Xu, M ;
Lydon, JP ;
O'Malley, BW ;
Levine, JE .
ENDOCRINOLOGY, 1999, 140 (08) :3653-3658
[9]   Stimulation of gonadotropin-releasing hormone surges by estrogen. I. Role of hypothalamic progesterone receptors [J].
Chappell, PE ;
Levine, JE .
ENDOCRINOLOGY, 2000, 141 (04) :1477-1485
[10]   Expression of ESR1 in Glutamatergic and GABAergic Neurons Is Essential for Normal Puberty Onset, Estrogen Feedback, and Fertility in Female Mice [J].
Cheong, Rachel Y. ;
Czieselsky, Katja ;
Porteous, Robert ;
Herbison, Allan E. .
JOURNAL OF NEUROSCIENCE, 2015, 35 (43) :14533-14543